Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F17%3A00095166" target="_blank" >RIV/00216224:14110/17:00095166 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_30" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-7021-6_30</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_30" target="_blank" >10.1007/978-1-4939-7021-6_30</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

Popis výsledku v původním jazyce

Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures

Název v anglickém jazyce

Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

Popis výsledku anglicky

Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

3D Cell Culture : Methods and Protocols

ISBN

9781493970193

ISSN

1064-3745

e-ISSN

—

Počet stran výsledku

15

Strana od-do

417-431

Název nakladatele

Humana Press

Místo vydání

New York

Místo konání akce

New York

Datum konání akce

1. 1. 2017

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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