Revealing 3D ultrastructure and morphology of stem cell spheroids by electron microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F17%3APU143838" target="_blank" >RIV/00216305:26620/17:PU143838 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.scopus.com/record/display.uri?eid=2-s2.0-85021355973&origin=resultslist&sort=plf-f&src=s&st1=Revealing+3D+ultrastructure+and+morphology+of+stem+cell+spheroids+by+electron+microscopy&sid=0976ed42b559776de22e21e63e60e432&sot=b&sdt=b&sl=103&s=TI" target="_blank" >https://www.scopus.com/record/display.uri?eid=2-s2.0-85021355973&origin=resultslist&sort=plf-f&src=s&st1=Revealing+3D+ultrastructure+and+morphology+of+stem+cell+spheroids+by+electron+microscopy&sid=0976ed42b559776de22e21e63e60e432&sot=b&sdt=b&sl=103&s=TI</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_30" target="_blank" >10.1007/978-1-4939-7021-6_30</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Revealing 3D ultrastructure and morphology of stem cell spheroids by electron microscopy

Popis výsledku v původním jazyce

Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures.

Název v anglickém jazyce

Revealing 3D ultrastructure and morphology of stem cell spheroids by electron microscopy

Popis výsledku anglicky

Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Methods in Molecular Biology

ISSN

1940-6029

e-ISSN

—

Svazek periodika

1612

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

417-431

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85021355973