Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00100860" target="_blank" >RIV/00216224:14110/18:00100860 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1292/jvms.17-0492" target="_blank" >http://dx.doi.org/10.1292/jvms.17-0492</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1292/jvms.17-0492" target="_blank" >10.1292/jvms.17-0492</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia
Popis výsledku v původním jazyce
Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity.
Název v anglickém jazyce
Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia
Popis výsledku anglicky
Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10601 - Cell biology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
JOURNAL OF VETERINARY MEDICAL SCIENCE
ISSN
0916-7250
e-ISSN
—
Svazek periodika
80
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
JP - Japonsko
Počet stran výsledku
9
Strana od-do
138-146
Kód UT WoS článku
000425785000023
EID výsledku v databázi Scopus
2-s2.0-85041730719