Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychos weddelli) in James Ross Island, Antarctica

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00084963" target="_blank" >RIV/00216224:14310/13:00084963 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychos weddelli) in James Ross Island, Antarctica

Popis výsledku v původním jazyce

A set of nine gram-negative fermenting rods biochemically identified as Escherichia coli was isolated from faeces of seals. These bacteria were characterized by phenotypic classification, 16S rDNA sequence analyses, automated ribotyping, study of whole-cell protein profiles by SDS-PAGE and finally by bacteriocin production. The results of our polyphasic taxonomic study supported the recognition of P4652, P4653 and P4740 isolates as true members of Escherichia albertii species ? probably a major enterichuman pathogen. To our best knowledge, this is the first evidence showing that E. albertii produces bacteriocin, and colicin D. Obtained data unambiguously showed inconvenience of commercial identification systems to distinguish both Escherichia speciesdue to missing data of E. albertii in the commercial databases. The results of Escherichia isolates taxonomy suggest seals as a novel source of human and animal pathogen, E. albertii in the Antarctic region.

Název v anglickém jazyce

Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychos weddelli) in James Ross Island, Antarctica

Popis výsledku anglicky

A set of nine gram-negative fermenting rods biochemically identified as Escherichia coli was isolated from faeces of seals. These bacteria were characterized by phenotypic classification, 16S rDNA sequence analyses, automated ribotyping, study of whole-cell protein profiles by SDS-PAGE and finally by bacteriocin production. The results of our polyphasic taxonomic study supported the recognition of P4652, P4653 and P4740 isolates as true members of Escherichia albertii species ? probably a major enterichuman pathogen. To our best knowledge, this is the first evidence showing that E. albertii produces bacteriocin, and colicin D. Obtained data unambiguously showed inconvenience of commercial identification systems to distinguish both Escherichia speciesdue to missing data of E. albertii in the commercial databases. The results of Escherichia isolates taxonomy suggest seals as a novel source of human and animal pathogen, E. albertii in the Antarctic region.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech polar Reports

ISSN

1805-0689

e-ISSN

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Svazek periodika

3

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

173-183

Kód UT WoS článku

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EID výsledku v databázi Scopus

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