TLR9 expression in the Choroid Plexus after subarachnoid hemorrhage

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

TLR9 expression in the Choroid Plexus after subarachnoid hemorrhage

Popis výsledku v původním jazyce

Subarachnoid hemorrhage (SAH) is a life-threatening emergency. The main noticeable changes after the onset of bleeding are reactive inflammatory reaction of neurons, glial cells and development of hydrocephalus. These changes are attributed to the effects of blood disintegration products, especially metabolites of heme. TLR9 may affect the tight junctions among the choroid plexus (CP) ependymal cells resulting in increased cerebrospinal fluid (CSF) production and increased intracranial pressure. Therefore, the aim of our study was to assess the changes of TLR9 in the CP after SAH. Wistar rats (n=40, males) were used in our experiments. Injection of autologous arterial blood into the cisterna magna was performed on experimental rats, while control animals received artificial CSF. The animals were left to survive for 1, 3 and 7 days from the application. Experimental and control rats were sacrificed together with naive rats and perfused transcardially with Zamboni´s fixative. Coronal cryostat sections through the brains were cut and immunostained for TLR9. The intensity of TLR9 immunofluorescence in the CP was quantified and statistically analyzed. The presence of TLR9 immunofluorescence was found in the cytoplasm of CP cuboidal cells in all animal groups. Statistically significant increase in TLR9 immunofluorescence was found in the CP of SAH animals in comparison to control and naive animals. This intensity increased with time of survival for 3 days. Our results clearly indicate that the CP reacts to SAH with an increase in TLR9 expression. These changes might be associated with increased intracranial pressure after SAH.

Název v anglickém jazyce

TLR9 expression in the Choroid Plexus after subarachnoid hemorrhage

Popis výsledku anglicky

Subarachnoid hemorrhage (SAH) is a life-threatening emergency. The main noticeable changes after the onset of bleeding are reactive inflammatory reaction of neurons, glial cells and development of hydrocephalus. These changes are attributed to the effects of blood disintegration products, especially metabolites of heme. TLR9 may affect the tight junctions among the choroid plexus (CP) ependymal cells resulting in increased cerebrospinal fluid (CSF) production and increased intracranial pressure. Therefore, the aim of our study was to assess the changes of TLR9 in the CP after SAH. Wistar rats (n=40, males) were used in our experiments. Injection of autologous arterial blood into the cisterna magna was performed on experimental rats, while control animals received artificial CSF. The animals were left to survive for 1, 3 and 7 days from the application. Experimental and control rats were sacrificed together with naive rats and perfused transcardially with Zamboni´s fixative. Coronal cryostat sections through the brains were cut and immunostained for TLR9. The intensity of TLR9 immunofluorescence in the CP was quantified and statistically analyzed. The presence of TLR9 immunofluorescence was found in the cytoplasm of CP cuboidal cells in all animal groups. Statistically significant increase in TLR9 immunofluorescence was found in the CP of SAH animals in comparison to control and naive animals. This intensity increased with time of survival for 3 days. Our results clearly indicate that the CP reacts to SAH with an increase in TLR9 expression. These changes might be associated with increased intracranial pressure after SAH.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů