Study of Hemeoxygenase-1 and Biliverdin Reductase expression in the Choroid Plexus after Subarachnoid Hemorrhage

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study of Hemeoxygenase-1 and Biliverdin Reductase expression in the Choroid Plexus after Subarachnoid Hemorrhage

Popis výsledku v původním jazyce

Subarachnoid hemorrhage (SAH) is a form of hemorrhagic stroke, associated with significant morbidity and mortality of affected patients. Complications after SAH include seizures, neurological deficits and an edematous state resulting from affected cerebrospinal fluid (CSF) circulatory dynamics. Blood clot degradation leads to activation of enzymes Hemeoxygenase-1 (HO-1) and Biliverdin reductase (BVR). The aim of our study was to describe the expression of HO-1 and BVR in the choroid plexus (CP) after SAH. Wistar rats (n=34; male) were used in our experiment. Injection of autologous arterial blood (SAH group) or artificial CSF (control group) to the cisterna magna was performed and rats were left to survive for 1, 3 and 7 days. SAH and control animals were sacrificed together with naive rats and coronal cryostat sections of the brain were prepared. Immunohistochemical detection of HO-1 and BVR enzymes in CP was performed, quantified and statistically analyzed. HO-1 was expressed in cuboidal cells (CC) and epiplexus cells (EC) in all groups of animals. After SAH, a higher statistically significant increase of HO-1 was observed in the CP compared with control and naive animals. Moreover, SAH induced increased number of HO-1+ EC following the time of survival. BVR was found in erythrocytes forming the clot on CP in animals 1 day after SAH. Subsequent survival times showed clot resolution and increased BVR expression in CC. An increased expression of HO-1 and BVR in CP was observed after SAH, suggesting possible disruption of CSF homeostasis and hydrocephalus formation.

Název v anglickém jazyce

Study of Hemeoxygenase-1 and Biliverdin Reductase expression in the Choroid Plexus after Subarachnoid Hemorrhage

Popis výsledku anglicky

Subarachnoid hemorrhage (SAH) is a form of hemorrhagic stroke, associated with significant morbidity and mortality of affected patients. Complications after SAH include seizures, neurological deficits and an edematous state resulting from affected cerebrospinal fluid (CSF) circulatory dynamics. Blood clot degradation leads to activation of enzymes Hemeoxygenase-1 (HO-1) and Biliverdin reductase (BVR). The aim of our study was to describe the expression of HO-1 and BVR in the choroid plexus (CP) after SAH. Wistar rats (n=34; male) were used in our experiment. Injection of autologous arterial blood (SAH group) or artificial CSF (control group) to the cisterna magna was performed and rats were left to survive for 1, 3 and 7 days. SAH and control animals were sacrificed together with naive rats and coronal cryostat sections of the brain were prepared. Immunohistochemical detection of HO-1 and BVR enzymes in CP was performed, quantified and statistically analyzed. HO-1 was expressed in cuboidal cells (CC) and epiplexus cells (EC) in all groups of animals. After SAH, a higher statistically significant increase of HO-1 was observed in the CP compared with control and naive animals. Moreover, SAH induced increased number of HO-1+ EC following the time of survival. BVR was found in erythrocytes forming the clot on CP in animals 1 day after SAH. Subsequent survival times showed clot resolution and increased BVR expression in CC. An increased expression of HO-1 and BVR in CP was observed after SAH, suggesting possible disruption of CSF homeostasis and hydrocephalus formation.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů