AgCu bimetallic nanoparticles modified by polyvinyl alcohol - the cells viability study in vitro
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00104068" target="_blank" >RIV/00216224:14110/18:00104068 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
AgCu bimetallic nanoparticles modified by polyvinyl alcohol - the cells viability study in vitro
Popis výsledku v původním jazyce
The effects of elementary metallic nanoparticles on living objects as cytotoxicity or antibacterial activity are widely known. Ag nanoparticles are a suitable and well known example. Nanoparticles formed by an alloy of Ag and Cu stabilized by polyvinyl alcohol were examined on two human tumor cells - ovarian carcinoma cells A2780 and skin melanoma cells A375. Bimetalic AgCu nanoparticles were synthetized by using a method of chemical co-reduction of silver nitrate and copper (II) nitrate hydrate. The nanoparticles were characterized by electron microscopy and by measurement of zeta potential. Cell viability was tested by using an MTT (tetrazole colorimetric viability assay) test. The effect on cell apoptosis and necrosis was measured by using flow cytometry. The experimental results indicate a differentiated impact of nanoparticles on the cells used. A more significant effect of viability decrease was observed for A2780 cells. The cell death caused by the nanoparticles used was observed particularly in the form of initial and advanced apoptosis for both cells lines, necrosis was observed to a lesser extent. The synthetized bimetallic nanoparticles seem to be a suitable candidate for targeted suppression of cell proliferation.
Název v anglickém jazyce
AgCu bimetallic nanoparticles modified by polyvinyl alcohol - the cells viability study in vitro
Popis výsledku anglicky
The effects of elementary metallic nanoparticles on living objects as cytotoxicity or antibacterial activity are widely known. Ag nanoparticles are a suitable and well known example. Nanoparticles formed by an alloy of Ag and Cu stabilized by polyvinyl alcohol were examined on two human tumor cells - ovarian carcinoma cells A2780 and skin melanoma cells A375. Bimetalic AgCu nanoparticles were synthetized by using a method of chemical co-reduction of silver nitrate and copper (II) nitrate hydrate. The nanoparticles were characterized by electron microscopy and by measurement of zeta potential. Cell viability was tested by using an MTT (tetrazole colorimetric viability assay) test. The effect on cell apoptosis and necrosis was measured by using flow cytometry. The experimental results indicate a differentiated impact of nanoparticles on the cells used. A more significant effect of viability decrease was observed for A2780 cells. The cell death caused by the nanoparticles used was observed particularly in the form of initial and advanced apoptosis for both cells lines, necrosis was observed to a lesser extent. The synthetized bimetallic nanoparticles seem to be a suitable candidate for targeted suppression of cell proliferation.
Klasifikace
Druh
J<sub>SC</sub> - Článek v periodiku v databázi SCOPUS
CEP obor
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OECD FORD obor
21001 - Nano-materials (production and properties)
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Lékař a technika
ISSN
0301-5491
e-ISSN
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Svazek periodika
48
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
6
Strana od-do
5-10
Kód UT WoS článku
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EID výsledku v databázi Scopus
2-s2.0-85049775759