TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F19%3A00107364" target="_blank" >RIV/00216224:14110/19:00107364 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/65269705:_____/19:00070655

Výsledek na webu

<a href="http://dx.doi.org/10.14735/amcsnn201953" target="_blank" >http://dx.doi.org/10.14735/amcsnn201953</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14735/amcsnn201953" target="_blank" >10.14735/amcsnn201953</a>

Jazyk výsledku

angličtina

Název v původním jazyce

TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage

Popis výsledku v původním jazyce

Aim: The aim of the study was to investigate expression changes of pro-inflammatory and proapoptotic cytokine tumor necrosis factor alpha (TNF alpha) and microRNAs (miRNAs) involved in its regulation in early pathophysiological changes after subarachnoid haemorrhage (SAH). Materials and methods: MiRNAs (miR-125b, miR-146a, miR-346, miR-155, miR-15b) and mRNA (TNF alpha) expression were determined by quantitative real-time polymerase chain reaction in brain tissue samples. A total of 88 animals were divided to Sham (control surgery without induction of SAH), Mild SAH, Severe SAH groups in following time-points: 2, 4, 6 and 8 h (n = 7 per group); including 4 animals used as an absolute control. Results: We have found a statistically significant difference in TNF alpha expression between Sham and Severe SAH groups at all the time-points (p &lt; 0.05), between Sham and Mild SAH groups 4 h after induction of SAH (p &lt; 0.05) and between Mild and Severe SAH groups at 2 and 6 h time-points (p &lt; 0.05). Furthermore, a significant difference in miR-15b expression between Sham and Severe SAH groups was observed 8 h after SAH (p &lt; 0.05). All the other microRNAs have not been significantly changed. Conclusions: SAH was associated with an early increase in TNF alpha and miR-15b expression especially in Severe SAH group. Despite complex cross-regulation between cytokines and miRNA, any information about the activation of inflammation/apoptotic mechanisms within a few hours after SAH may improve our knowledge of SAH pathophysiology. Furthermore, it can lead to therapeutic improvement using a combination of both pro-apoptotic markers TNF alpha and miR-15b.

Název v anglickém jazyce

TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage

Popis výsledku anglicky

Aim: The aim of the study was to investigate expression changes of pro-inflammatory and proapoptotic cytokine tumor necrosis factor alpha (TNF alpha) and microRNAs (miRNAs) involved in its regulation in early pathophysiological changes after subarachnoid haemorrhage (SAH). Materials and methods: MiRNAs (miR-125b, miR-146a, miR-346, miR-155, miR-15b) and mRNA (TNF alpha) expression were determined by quantitative real-time polymerase chain reaction in brain tissue samples. A total of 88 animals were divided to Sham (control surgery without induction of SAH), Mild SAH, Severe SAH groups in following time-points: 2, 4, 6 and 8 h (n = 7 per group); including 4 animals used as an absolute control. Results: We have found a statistically significant difference in TNF alpha expression between Sham and Severe SAH groups at all the time-points (p &lt; 0.05), between Sham and Mild SAH groups 4 h after induction of SAH (p &lt; 0.05) and between Mild and Severe SAH groups at 2 and 6 h time-points (p &lt; 0.05). Furthermore, a significant difference in miR-15b expression between Sham and Severe SAH groups was observed 8 h after SAH (p &lt; 0.05). All the other microRNAs have not been significantly changed. Conclusions: SAH was associated with an early increase in TNF alpha and miR-15b expression especially in Severe SAH group. Despite complex cross-regulation between cytokines and miRNA, any information about the activation of inflammation/apoptotic mechanisms within a few hours after SAH may improve our knowledge of SAH pathophysiology. Furthermore, it can lead to therapeutic improvement using a combination of both pro-apoptotic markers TNF alpha and miR-15b.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

<a href="/cs/project/GP14-23773P" target="_blank" >GP14-23773P: Regulace zánětlivé odpovědi prostřednictvím mikroRNA na experimaentálním modelu subarachnoidálního krvácení</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Česká a slovenská neurologie a neurochirurgie

ISSN

1210-7859

e-ISSN

—

Svazek periodika

82

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

53-59

Kód UT WoS článku

000458015800007

EID výsledku v databázi Scopus

2-s2.0-85066988260