Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F20%3A00116132" target="_blank" >RIV/00216224:14110/20:00116132 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/1422-0067/21/13/4728" target="_blank" >https://www.mdpi.com/1422-0067/21/13/4728</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21134728" target="_blank" >10.3390/ijms21134728</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis

Popis výsledku v původním jazyce

Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional variants in selected interleukin (IL) genes in patients with the generalized form of AgP in a homogenous population. Twelve polymorphisms inIL-1gene cluster,IL-6and its receptor,IL-10,IL-17A, andIL-18were determined in 91 AgP patients and 210 controls. Analysis of seven selected periodontal bacteria in subgingival sulci/pockets was performed with a commercial DNA-microarray kit in a subgroup of 76 individuals. The pilot in vitro study included stimulation of peripheral blood monocytes (PBMC) from 20 individuals with periodontal bacteria and measurement of IL-10 levels using the Luminex method. Only the unctional polymorphismIL-10-1087 A/G (rs1800896) and specificIL-10haplotypes were associated with the development of the disease (p&lt; 0.05,P-corr&gt; 0.05). Four bacterial species occurred more frequently in AgP than in controls (p&lt; 0.01,P-corr&lt; 0.05). Elevated IL-10 levels were found in AgP patients, carriers ofIL-10-1087GG genotype, and PBMCs stimulated by periodontal bacteria (p&lt; 0.05,P-corr&gt; 0.05). We therefore conclude that a combination of genetic predisposition to the altered expression ofIL-10and the presence of specific periodontal bacteria may contribute to Th1/Th2 balance disruption and AgP development.

Název v anglickém jazyce

Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis

Popis výsledku anglicky

Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional variants in selected interleukin (IL) genes in patients with the generalized form of AgP in a homogenous population. Twelve polymorphisms inIL-1gene cluster,IL-6and its receptor,IL-10,IL-17A, andIL-18were determined in 91 AgP patients and 210 controls. Analysis of seven selected periodontal bacteria in subgingival sulci/pockets was performed with a commercial DNA-microarray kit in a subgroup of 76 individuals. The pilot in vitro study included stimulation of peripheral blood monocytes (PBMC) from 20 individuals with periodontal bacteria and measurement of IL-10 levels using the Luminex method. Only the unctional polymorphismIL-10-1087 A/G (rs1800896) and specificIL-10haplotypes were associated with the development of the disease (p&lt; 0.05,P-corr&gt; 0.05). Four bacterial species occurred more frequently in AgP than in controls (p&lt; 0.01,P-corr&lt; 0.05). Elevated IL-10 levels were found in AgP patients, carriers ofIL-10-1087GG genotype, and PBMCs stimulated by periodontal bacteria (p&lt; 0.05,P-corr&gt; 0.05). We therefore conclude that a combination of genetic predisposition to the altered expression ofIL-10and the presence of specific periodontal bacteria may contribute to Th1/Th2 balance disruption and AgP development.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/NU20-08-00205" target="_blank" >NU20-08-00205: Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1661-6596

e-ISSN

1422-0067

Svazek periodika

21

Číslo periodika v rámci svazku

13

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

24

Strana od-do

1-24

Kód UT WoS článku

000550282500001

EID výsledku v databázi Scopus

2-s2.0-85087462313