IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID

Popis výsledku v původním jazyce

Common variable immunodeficiency disorder (CVID) is the most common form of clinically significant primary immunodeficiency. Majority of patients receive intravenous immunoglobulin (IVIg) replacement therapy with potent immunomodulatory properties on individual components of the immune system. CVID is associated with chronic granulocytic activation and an increased percentage of Low Density Neutrophils (LDN) observed in the layer of peripheral blood mononuclear cells (PBMC). Changes in the percentage of LDN in PBMC and expression of their surface markers were studied in 25 CVID patients and 27 healthy donors (HD) in a whole blood sample and after in-vitro stimulation of whole blood with IVIg. We confirmed an increased number of LDN in the PBMC layer in a fresh blood sample from CVID patients. The LDN of patients and a HD group consisted of mature and immature neutrophils distinguished based on the expression of CD10 and CD16. Stimulation of whole blood with IVIg caused an increase of LDN in both CVID patients and HD. This enhancement in LDN was caused by mature neutrophils CD16+CD10+ in both groups. The suppressive effect of induced LDN on T-cell proliferation was confirmed. Flow cytometry measurements revealed altered expression of CD274 and CD10 on mature LDN of CVID patients. The addition of IVIg inhibited the expression of CD11b on the mature LDN in both experimental groups. These results indicate that IVIg supports the formation of LDN, which can negatively affect the immune response and can significantly contribute to the chronic inflammation observed in patients with CVID.

Název v anglickém jazyce

IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID

Popis výsledku anglicky

Common variable immunodeficiency disorder (CVID) is the most common form of clinically significant primary immunodeficiency. Majority of patients receive intravenous immunoglobulin (IVIg) replacement therapy with potent immunomodulatory properties on individual components of the immune system. CVID is associated with chronic granulocytic activation and an increased percentage of Low Density Neutrophils (LDN) observed in the layer of peripheral blood mononuclear cells (PBMC). Changes in the percentage of LDN in PBMC and expression of their surface markers were studied in 25 CVID patients and 27 healthy donors (HD) in a whole blood sample and after in-vitro stimulation of whole blood with IVIg. We confirmed an increased number of LDN in the PBMC layer in a fresh blood sample from CVID patients. The LDN of patients and a HD group consisted of mature and immature neutrophils distinguished based on the expression of CD10 and CD16. Stimulation of whole blood with IVIg caused an increase of LDN in both CVID patients and HD. This enhancement in LDN was caused by mature neutrophils CD16+CD10+ in both groups. The suppressive effect of induced LDN on T-cell proliferation was confirmed. Flow cytometry measurements revealed altered expression of CD274 and CD10 on mature LDN of CVID patients. The addition of IVIg inhibited the expression of CD11b on the mature LDN in both experimental groups. These results indicate that IVIg supports the formation of LDN, which can negatively affect the immune response and can significantly contribute to the chronic inflammation observed in patients with CVID.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30102 - Immunology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů