Proteomic profiles of patients suffering from subarachnoid hemorrhage (WFNS Dubai)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F25%3A00142952" target="_blank" >RIV/00216224:14110/25:00142952 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Proteomic profiles of patients suffering from subarachnoid hemorrhage (WFNS Dubai)

Popis výsledku v původním jazyce

Background Inflammation is an important contributor to early brain injury after subarachnoid hemorrhage (SAH). IL-6 represents the most frequently studied marker of SAH-associated inflammation. However, IL-6 levels after SAH are highly variable, and therefore, it is not possible to define diagnostically relevant threshold levels. Our project aims to identify biomarkers associated with the variability of IL-6 using proteomic analysis. Methods Patients with a diagnosis of SAH, who were admitted to the Department of Neurosurgery within 24 hours after the onset of bleeding were enrolled in this study. All the treatments were standard, including treatment of aneurysm (surgery or endovascular treatment). Additionally, the samples of blood and CSF were collected at intervals of 24 hours from day 1 to day 3 after SAH. IL-6 levels in CSF were evaluated using ELISA in all the samples. Proteomic analyses were performed by liquid chromatography-mass spectrometry using a bottom-up approach with respect to IL-6 levels in CSF. Results This poster summarizes the primary results that characterize SAH-associated proteomic changes in blood and CSF in 29 SAH patients. There are 15 females and 14 males, and the mean age of the study group is 55,03 years. Most patients suffered from mild/moderate SAH (WFNS 1 – 10 patients, 2 – 4 patients, 3 -6 patients, 4 -1 patients, 5- 8 patients). Seven patients underwent surgical treatment, four patients were treated by endovascular procedures, and the source of bleeding was not proven in two cases. The preliminary results indicate the upregulation of processes related to inflammation, apoptosis, and coagulation. Several candidate proteins including IG heavy chain protein, Phosphatidylinositol-glycan-specific phospholipase D, EGF containing fibulin extracellular matrix protein 1 or Coagulation factor 11 protein will be the subject of further research concerning IL-6 expression. Conclusions Processes related to inflammation, apoptosis and coagulation cascade are promising candidates for research of pathophysiology of SAH. Deeper understanding of these processes may lead to identification of new diagnostic markers, which may be useful in treatment of SAH patients.

Název v anglickém jazyce

Proteomic profiles of patients suffering from subarachnoid hemorrhage (WFNS Dubai)

Popis výsledku anglicky

Background Inflammation is an important contributor to early brain injury after subarachnoid hemorrhage (SAH). IL-6 represents the most frequently studied marker of SAH-associated inflammation. However, IL-6 levels after SAH are highly variable, and therefore, it is not possible to define diagnostically relevant threshold levels. Our project aims to identify biomarkers associated with the variability of IL-6 using proteomic analysis. Methods Patients with a diagnosis of SAH, who were admitted to the Department of Neurosurgery within 24 hours after the onset of bleeding were enrolled in this study. All the treatments were standard, including treatment of aneurysm (surgery or endovascular treatment). Additionally, the samples of blood and CSF were collected at intervals of 24 hours from day 1 to day 3 after SAH. IL-6 levels in CSF were evaluated using ELISA in all the samples. Proteomic analyses were performed by liquid chromatography-mass spectrometry using a bottom-up approach with respect to IL-6 levels in CSF. Results This poster summarizes the primary results that characterize SAH-associated proteomic changes in blood and CSF in 29 SAH patients. There are 15 females and 14 males, and the mean age of the study group is 55,03 years. Most patients suffered from mild/moderate SAH (WFNS 1 – 10 patients, 2 – 4 patients, 3 -6 patients, 4 -1 patients, 5- 8 patients). Seven patients underwent surgical treatment, four patients were treated by endovascular procedures, and the source of bleeding was not proven in two cases. The preliminary results indicate the upregulation of processes related to inflammation, apoptosis, and coagulation. Several candidate proteins including IG heavy chain protein, Phosphatidylinositol-glycan-specific phospholipase D, EGF containing fibulin extracellular matrix protein 1 or Coagulation factor 11 protein will be the subject of further research concerning IL-6 expression. Conclusions Processes related to inflammation, apoptosis and coagulation cascade are promising candidates for research of pathophysiology of SAH. Deeper understanding of these processes may lead to identification of new diagnostic markers, which may be useful in treatment of SAH patients.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30212 - Surgery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů