Activation of Wnt/β-catenin signaling is critical for the tumorigenesis of choroid plexus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F25%3A00144241" target="_blank" >RIV/00216224:14110/25:00144241 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/neuro-oncology/advance-article/doi/10.1093/neuonc/noae176/7746517?login=true" target="_blank" >https://academic.oup.com/neuro-oncology/advance-article/doi/10.1093/neuonc/noae176/7746517?login=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/neuonc/noae176" target="_blank" >10.1093/neuonc/noae176</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Activation of Wnt/β-catenin signaling is critical for the tumorigenesis of choroid plexus

Popis výsledku v původním jazyce

Background. The choroid plexus (ChP) is the secretory epithelial structure located in the brain ventricles. Choroid plexus tumors (CPTs) are rare neoplasms predominantly occurring in young patients with intensified malignancy in children. CPT treatment is hindered by insufficient knowledge of tumor pathology and the limited availability of valid models. Methods. Genomic and transcriptomic data from CPT patients were analyzed to identify the putative pathological pathway. Cellular and molecular techniques were employed to validate bioinformatic results in CPT patient samples. Pharmacologic inhibition of Wnt/beta-catenin signaling was assessed in CPT cells. Cell-based assays of ChP cell lines were performed following CRISPR-Cas9-derived knockout and overexpression of Wnt/beta-catenin pathway genes. A 3D CPT model was generated through CRISPR-Cas9-derived knockout of APC. Results. We discovered that Wnt/beta-catenin signaling is activated in human CPTs, likely as a consequence of large-scale chromosomal instability events of the CPT genomes. We demonstrated that CPT-derived cells depend on autocrine Wnt/beta-catenin signaling for survival. Constitutive Wnt/beta-catenin pathway activation, either through knockout of the negative regulator APC or overexpression of the ligand WNT3A, induced tumorigenic properties in ChP 2D in vitro models. Increased activation of the Wnt/beta-catenin pathway in ChP organoids, through treatment with a potent GSK3 beta inhibitor, reduced the differentiation of mature ChP epithelial cells. Remarkably, the depletion of APC was sufficient to induce the oncogenic transformation of ChP organoids. Conclusions. Our research identifies Wnt/beta-catenin signaling as a critical driver of CPT tumorigenesis and provides the first 3D in vitro model for future pathological and therapeutic studies of CPT.

Název v anglickém jazyce

Activation of Wnt/β-catenin signaling is critical for the tumorigenesis of choroid plexus

Popis výsledku anglicky

Background. The choroid plexus (ChP) is the secretory epithelial structure located in the brain ventricles. Choroid plexus tumors (CPTs) are rare neoplasms predominantly occurring in young patients with intensified malignancy in children. CPT treatment is hindered by insufficient knowledge of tumor pathology and the limited availability of valid models. Methods. Genomic and transcriptomic data from CPT patients were analyzed to identify the putative pathological pathway. Cellular and molecular techniques were employed to validate bioinformatic results in CPT patient samples. Pharmacologic inhibition of Wnt/beta-catenin signaling was assessed in CPT cells. Cell-based assays of ChP cell lines were performed following CRISPR-Cas9-derived knockout and overexpression of Wnt/beta-catenin pathway genes. A 3D CPT model was generated through CRISPR-Cas9-derived knockout of APC. Results. We discovered that Wnt/beta-catenin signaling is activated in human CPTs, likely as a consequence of large-scale chromosomal instability events of the CPT genomes. We demonstrated that CPT-derived cells depend on autocrine Wnt/beta-catenin signaling for survival. Constitutive Wnt/beta-catenin pathway activation, either through knockout of the negative regulator APC or overexpression of the ligand WNT3A, induced tumorigenic properties in ChP 2D in vitro models. Increased activation of the Wnt/beta-catenin pathway in ChP organoids, through treatment with a potent GSK3 beta inhibitor, reduced the differentiation of mature ChP epithelial cells. Remarkably, the depletion of APC was sufficient to induce the oncogenic transformation of ChP organoids. Conclusions. Our research identifies Wnt/beta-catenin signaling as a critical driver of CPT tumorigenesis and provides the first 3D in vitro model for future pathological and therapeutic studies of CPT.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/GA21-21612S" target="_blank" >GA21-21612S: Souhra mezi regulacemi buněčného cyklu a ciliogeneze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neuro-Oncology

ISSN

1522-8517

e-ISSN

1523-5866

Svazek periodika

27

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

17

Strana od-do

106-122

Kód UT WoS článku

001325265000001

EID výsledku v databázi Scopus

2-s2.0-85215425092