STUDY OF THE EFFECT OF PARACETAMOL BINDED IN POLYMERIC NANOPARTICLES ON DAFNIA MAGNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14160%2F21%3A00129821" target="_blank" >RIV/00216224:14160/21:00129821 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.confer.cz/nanocon/2020/3747-study-of-the-effect-of-paracetamol-binded-in-polymeric-nanoparticles-on-dafnia-magna" target="_blank" >https://www.confer.cz/nanocon/2020/3747-study-of-the-effect-of-paracetamol-binded-in-polymeric-nanoparticles-on-dafnia-magna</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2020.3747" target="_blank" >10.37904/nanocon.2020.3747</a>

Jazyk výsledku

angličtina

Název v původním jazyce

STUDY OF THE EFFECT OF PARACETAMOL BINDED IN POLYMERIC NANOPARTICLES ON DAFNIA MAGNA

Popis výsledku v původním jazyce

Drugs are important xenobiotics in the environment. Their use increases with the growth of the human population, but also in agricultural primary production. Paracetamol (PAR) is a widely used analgesic and antipyretic and its production is still growing. Commonly available drug production technologies are being developed very intensively with nanotechnological modifications for their gradual and targeted release. Nanoparticles (ST/PAR) from starch were prepared: PAR (0, 1, 2, 3, 5 and 10 mg/L) was mixed with citric acid ester in a 1:8 v/v ratio for 30 min at 25 degrees C. By the centrifugation (16.000 g, 30 min) ST/PAR were obtained in the pellet. The effect of PAR was studied on Daphnia magna Straus (Cladocera, Crustacea). Adult females (70-400 mg) were used for self-evaluation. The EC50 was 3.749 mg/L after 48 h of PAR treatment. Total protein values determined by Lowry method were between 0.5-2.2 mg/mL and by Bradford method between 190-676 mg/L. Antioxidant activity values determined by CUPRAC method were between 4-15 mu g/mL GAE and by ABTS method ranged between 40-103 mu g/mL GAE. PAR values were between 9-40 mu M. Subsequently, the biological activity of the prepared nanoparticles was tested.

Název v anglickém jazyce

STUDY OF THE EFFECT OF PARACETAMOL BINDED IN POLYMERIC NANOPARTICLES ON DAFNIA MAGNA

Popis výsledku anglicky

Drugs are important xenobiotics in the environment. Their use increases with the growth of the human population, but also in agricultural primary production. Paracetamol (PAR) is a widely used analgesic and antipyretic and its production is still growing. Commonly available drug production technologies are being developed very intensively with nanotechnological modifications for their gradual and targeted release. Nanoparticles (ST/PAR) from starch were prepared: PAR (0, 1, 2, 3, 5 and 10 mg/L) was mixed with citric acid ester in a 1:8 v/v ratio for 30 min at 25 degrees C. By the centrifugation (16.000 g, 30 min) ST/PAR were obtained in the pellet. The effect of PAR was studied on Daphnia magna Straus (Cladocera, Crustacea). Adult females (70-400 mg) were used for self-evaluation. The EC50 was 3.749 mg/L after 48 h of PAR treatment. Total protein values determined by Lowry method were between 0.5-2.2 mg/mL and by Bradford method between 190-676 mg/L. Antioxidant activity values determined by CUPRAC method were between 4-15 mu g/mL GAE and by ABTS method ranged between 40-103 mu g/mL GAE. PAR values were between 9-40 mu M. Subsequently, the biological activity of the prepared nanoparticles was tested.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings 12th International Conference on Nanomaterials - Research & Application

ISBN

9788087294987

ISSN

2694-930X

e-ISSN

—

Počet stran výsledku

6

Strana od-do

318-323

Název nakladatele

TANGER LTD

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

21. 10. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000664505500054