PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14160%2F21%3A00133494" target="_blank" >RIV/00216224:14160/21:00133494 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.confer.cz/nanocon/2020/3746-preparation-of-silver-nanoparticles-prepared-by-green-synthesis-of-vine-seeds-antibacterial-effect" target="_blank" >https://www.confer.cz/nanocon/2020/3746-preparation-of-silver-nanoparticles-prepared-by-green-synthesis-of-vine-seeds-antibacterial-effect</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2020.3746" target="_blank" >10.37904/nanocon.2020.3746</a>

Jazyk výsledku

angličtina

Název v původním jazyce

PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT

Popis výsledku v původním jazyce

Several silver nanoparticles (AgNPs) with biological effect were prepared by green synthesis. AgNPs were prepared from Vitis vinifera seeds (Merlos variety) used as wine production waste. Green synthesis brings intensive surface modifications of nanoparticles. The seeds were lyophilized and then homogeneously ground. Purified extracts (0.5, 2.5, 5 and 10 g / 100 mL water) were added to AgNO3 solution(1 M) in a 1:1(v/v) ratio and stirred for 24 h. The formed nanoparticles were precipitated with methanol (1:1) (v/v) and lyophilized. Secondary metabolites were analyzed by various methods. The preparation yield of AgNPs ranged between 3 - 25 %. The total protein values determined by the Lowry method ranged between 1.8-3.6 mg/mL. Antioxidant activity values determined by the CUPRAC method ranged between 76 - 157 mu g/mL GAE. Total phenols values determined by Folin-Ciocalteu ranged between 88 - 160 mu g/mL GAE, by 4-aminoantipyrine method (TAAP) ranged between 728 - 1,299 mu g/mL GAE, by Price Butler method (PBM) ranged between 57 - 108 mu g/mL GAE. AgNPs prepared from Vitis vinifera seeds (AgNPs-VV) showed also antibacterial activity. Minimal inhibition concentration (MIC) of AgNPs-VV in S.aureus was 31 mu g/mL and MIC in E. coli was 55 mu g/mL.

Název v anglickém jazyce

PREPARATION OF SILVER NANOPARTICLES BY GREEN SYNTHESIS OF VINE SEEDS - ANTIBACTERIAL EFFECT

Popis výsledku anglicky

Several silver nanoparticles (AgNPs) with biological effect were prepared by green synthesis. AgNPs were prepared from Vitis vinifera seeds (Merlos variety) used as wine production waste. Green synthesis brings intensive surface modifications of nanoparticles. The seeds were lyophilized and then homogeneously ground. Purified extracts (0.5, 2.5, 5 and 10 g / 100 mL water) were added to AgNO3 solution(1 M) in a 1:1(v/v) ratio and stirred for 24 h. The formed nanoparticles were precipitated with methanol (1:1) (v/v) and lyophilized. Secondary metabolites were analyzed by various methods. The preparation yield of AgNPs ranged between 3 - 25 %. The total protein values determined by the Lowry method ranged between 1.8-3.6 mg/mL. Antioxidant activity values determined by the CUPRAC method ranged between 76 - 157 mu g/mL GAE. Total phenols values determined by Folin-Ciocalteu ranged between 88 - 160 mu g/mL GAE, by 4-aminoantipyrine method (TAAP) ranged between 728 - 1,299 mu g/mL GAE, by Price Butler method (PBM) ranged between 57 - 108 mu g/mL GAE. AgNPs prepared from Vitis vinifera seeds (AgNPs-VV) showed also antibacterial activity. Minimal inhibition concentration (MIC) of AgNPs-VV in S.aureus was 31 mu g/mL and MIC in E. coli was 55 mu g/mL.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH &amp; APPLICATION (NANOCON 2020)

ISBN

9788087294987

ISSN

2694-930X

e-ISSN

—

Počet stran výsledku

6

Strana od-do

422-427

Název nakladatele

TANGER LTD

Místo vydání

SLEZSKA

Místo konání akce

Brno, CZECH REPUBLIC

Datum konání akce

1. 2. 2020

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000664505500072