Green synthesis of silver nanoparticles using sage and their antibacterial effect on gram-positive and gram-negative bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F20%3A43917982" target="_blank" >RIV/62156489:43510/20:43917982 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.37904/nanocon.2019.8573" target="_blank" >https://doi.org/10.37904/nanocon.2019.8573</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2019.8573" target="_blank" >10.37904/nanocon.2019.8573</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Green synthesis of silver nanoparticles using sage and their antibacterial effect on gram-positive and gram-negative bacteria

Popis výsledku v původním jazyce

In this work, we focused on the green synthesis of silver nanoparticles (AgNPs). In the green synthesis, plant extracts are used as reducing agents. AgNPs contain surface-bound biomolecules from the used plant extract. We used an aqueous extract from sage (Salvia officinalis). This plant is known for its high content of secondary metabolites with excellent antibacterial activity. Antibacterial activity of AgNPs prepared by green synthesis was investigated. Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli were selected for testing. Methods such as agar diffusion assay and inhibition of bacterial growth curves were used. Minimum inhibitory concentrations (MICE. coli = 250 µg/mL; MICS. aureus = 50 µg/mL) were determined. The radiuses of inhibition zones were about 1.5 cm in both bacterial strains. AgNPs prepared by this method can play an important role in dealing with the problem of bacterial resistance. Bacteria develop resistance not only to typical antibiotics. Bacterial cells are also able to eliminate the effect of AgNPs. By modifying their surface with antibacterial molecules, resistance can be effectively combatted.

Název v anglickém jazyce

Green synthesis of silver nanoparticles using sage and their antibacterial effect on gram-positive and gram-negative bacteria

Popis výsledku anglicky

In this work, we focused on the green synthesis of silver nanoparticles (AgNPs). In the green synthesis, plant extracts are used as reducing agents. AgNPs contain surface-bound biomolecules from the used plant extract. We used an aqueous extract from sage (Salvia officinalis). This plant is known for its high content of secondary metabolites with excellent antibacterial activity. Antibacterial activity of AgNPs prepared by green synthesis was investigated. Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli were selected for testing. Methods such as agar diffusion assay and inhibition of bacterial growth curves were used. Minimum inhibitory concentrations (MICE. coli = 250 µg/mL; MICS. aureus = 50 µg/mL) were determined. The radiuses of inhibition zones were about 1.5 cm in both bacterial strains. AgNPs prepared by this method can play an important role in dealing with the problem of bacterial resistance. Bacteria develop resistance not only to typical antibiotics. Bacterial cells are also able to eliminate the effect of AgNPs. By modifying their surface with antibacterial molecules, resistance can be effectively combatted.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/LTC18002" target="_blank" >LTC18002: Vývoj nových materiálů vhodných pro 3D tisk s antimikrobiálními vlastnostmi (3D ANTIMICROB)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2019: Conference Proceedings

ISBN

978-80-87294-95-6

ISSN

2694-930X

e-ISSN

—

Počet stran výsledku

5

Strana od-do

469-473

Název nakladatele

Tanger Ltd.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

16. 10. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000664115400080