Silver nanoparticles from the sage (AgNPs) and their biological effect on S. aureus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F19%3A43920914" target="_blank" >RIV/62156489:43510/19:43920914 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Silver nanoparticles from the sage (AgNPs) and their biological effect on S. aureus

Popis výsledku v původním jazyce

Silver (Ag&apos;) ions and several compounds containing silver ions are highly toxic to microorganisms and exhibit strong biocidal effects on many species of bacteria however may be harmful to the environment and act as a carcinogen. Using silver nanopartieles instead of bulk silver, yields increased antibacterial activity at reduced toxic concentration [ I ]. Thus, the optimization of silver nanopartieles as antibacterial agents is important task, especially for multidrug resistant bacteria. Sage (Salvia officinalis) is used in traditional medicine for its antibacterial effects 12,31. Extracts of this plant arc suitable substrates for the preparation of silver nanopartieles. Biomolecules contained in the plant extract bond with the surface of AgNPs. These biomolecules improve biological activity of AgNPs which can be used to treat infections caused by antibiotic resistant bacteria. The effect of the sage nanopartieles prepared by green synthesis on wild-type strain cells was studied. In our experiment. 4 different amounts (50. 100, 150 and 200 pg of AgNPs/mL) produced at 4 different temperatures (20, 40, 60 and 80 oC) were pipetted onto the Petri dishes. The best antibacterial effect to S. aureus and E. coli was found with the AgNPs prepared by using the extract which was prepared by 20 and 60 oC. Silver effect on S. aureus was lower, than on Gram-negative bacteria. MIC of silver ions on S. aureus cells was observed as 50 ug/ml. Then, inhibition zones by agar diffusion test were observed. Petri dishes with agar medium were covered by 50 pL of bacteria suspension (OD620 = 0.01). AgNPs (c = 10 mg/mL) dispersion was pipetted into a Petri dish in amounts of 5, 10, 15 and 20 pL. This resulted in variants with concentrations of 50, 100, 150 and of 200 pg/mL. The bacteria were cultivated for 12 h at 37 oC. After 12 h of incubation, photographs were taken, and the total area of the inhibition zone in cm2 was calculated in the LIS programme. When the inhibition zone began to overgrow with bacterial colonies, a constant value of 0.01 cm2 was selected. Inhibitor zone sizes ranged from 0.01 cm2 to 1.5 cm2. The best antibacterial effect on S. aureus was found with the AgNPs prepared by using the extract that was prepared at 20 oC (value 0.9 cm2 at concentration 200 pg/mL). The best effect on E. coli also had AgNPs prepared by using the extract at 20 oC (value 1.5 cm2 at concentration 200 pg/mL). A better antibacterial effect of AgNPs was obtained against E. coli. In this experimental work antibacterial effect of AgNPs prepared by green synthesis was observed. Two different microbiological methods were used. Great antibacterial activity of AgNPs to G+ and G- bacteria, was proved. Green synthesis prepared nanopartieles have a lot of preferences, firstly, lower toxicity than other silver-contained drugs with strongest effect and has a large potential for using instead of antibiotics.

Název v anglickém jazyce

Silver nanoparticles from the sage (AgNPs) and their biological effect on S. aureus

Popis výsledku anglicky

Silver (Ag&apos;) ions and several compounds containing silver ions are highly toxic to microorganisms and exhibit strong biocidal effects on many species of bacteria however may be harmful to the environment and act as a carcinogen. Using silver nanopartieles instead of bulk silver, yields increased antibacterial activity at reduced toxic concentration [ I ]. Thus, the optimization of silver nanopartieles as antibacterial agents is important task, especially for multidrug resistant bacteria. Sage (Salvia officinalis) is used in traditional medicine for its antibacterial effects 12,31. Extracts of this plant arc suitable substrates for the preparation of silver nanopartieles. Biomolecules contained in the plant extract bond with the surface of AgNPs. These biomolecules improve biological activity of AgNPs which can be used to treat infections caused by antibiotic resistant bacteria. The effect of the sage nanopartieles prepared by green synthesis on wild-type strain cells was studied. In our experiment. 4 different amounts (50. 100, 150 and 200 pg of AgNPs/mL) produced at 4 different temperatures (20, 40, 60 and 80 oC) were pipetted onto the Petri dishes. The best antibacterial effect to S. aureus and E. coli was found with the AgNPs prepared by using the extract which was prepared by 20 and 60 oC. Silver effect on S. aureus was lower, than on Gram-negative bacteria. MIC of silver ions on S. aureus cells was observed as 50 ug/ml. Then, inhibition zones by agar diffusion test were observed. Petri dishes with agar medium were covered by 50 pL of bacteria suspension (OD620 = 0.01). AgNPs (c = 10 mg/mL) dispersion was pipetted into a Petri dish in amounts of 5, 10, 15 and 20 pL. This resulted in variants with concentrations of 50, 100, 150 and of 200 pg/mL. The bacteria were cultivated for 12 h at 37 oC. After 12 h of incubation, photographs were taken, and the total area of the inhibition zone in cm2 was calculated in the LIS programme. When the inhibition zone began to overgrow with bacterial colonies, a constant value of 0.01 cm2 was selected. Inhibitor zone sizes ranged from 0.01 cm2 to 1.5 cm2. The best antibacterial effect on S. aureus was found with the AgNPs prepared by using the extract that was prepared at 20 oC (value 0.9 cm2 at concentration 200 pg/mL). The best effect on E. coli also had AgNPs prepared by using the extract at 20 oC (value 1.5 cm2 at concentration 200 pg/mL). A better antibacterial effect of AgNPs was obtained against E. coli. In this experimental work antibacterial effect of AgNPs prepared by green synthesis was observed. Two different microbiological methods were used. Great antibacterial activity of AgNPs to G+ and G- bacteria, was proved. Green synthesis prepared nanopartieles have a lot of preferences, firstly, lower toxicity than other silver-contained drugs with strongest effect and has a large potential for using instead of antibiotics.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LTC18002" target="_blank" >LTC18002: Vývoj nových materiálů vhodných pro 3D tisk s antimikrobiálními vlastnostmi (3D ANTIMICROB)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů