Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14160%2F22%3A00119771" target="_blank" >RIV/00216224:14160/22:00119771 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12520/22:43904613

Výsledek na webu

<a href="https://www.mdpi.com/2076-2615/12/2/208" target="_blank" >https://www.mdpi.com/2076-2615/12/2/208</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani12020208" target="_blank" >10.3390/ani12020208</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles

Popis výsledku v původním jazyce

Simple Summary Pikeperch Sander lucioperca is a promising candidate for intensive aquaculture. However, controlled reproduction has become the major bottleneck in pikeperch production. To improve and optimize its artificial reproduction, an effective method for hormone treatment is needed. The use of a poly(lactic-co-glycolic acid) microparticle-sustained-release system to administer gonadotropin-releasing hormone agonist to pikeperch resulted in acceptable reproductive output. Our results establish the potential of poly(lactic-co-glycolic acid) microparticle as a novel tool for hormone treatment in fish. Pikeperch Sander lucioperca is a piscivorous species considered a promising candidate for the diversification of intensive aquaculture. This study aimed to determine the effect of a sustained-release delivery system incorporating mammalian gonadotropin-releasing hormone agonist (mGnRHa) into poly(lactic-co-glycolic acid) (PLGA) microparticles on the sex steroid levels and aspects of artificial reproduction of pikeperch. Fish were divided into four groups and injected with 20 mu g mGnRHa/kg, 5-day release microparticles encapsulated with 5 mu g GnRHa/kg BW (PLGA 5), 20 mu g GnRHa/kg (PLGA 20), or 1 mL/kg 0.9% NaCl (control). Cumulative percentage ovulation was 100% in the PLGA 5 group, significantly higher than in other tested groups. No differences among groups were observed in latency or fecundity. The level of 11-ketotestosterone (11-KT) peaked at 40 h post-injection, and was sustained during ovulation, in all treated groups. The 17 beta-estradiol (E2) concentration increased in the mGnRHa-only group immediately after hormone injection, while both PLGA groups showed a reduction in E2 after injection, continuing to decrease until ovulation. A low dose of mGnRHa in PLGA microparticles significantly improves induction of ovulation and results in acceptable reproductive performance, which may positively affect pikeperch production under controlled conditions.

Název v anglickém jazyce

Spawning Performance and Sex Steroid Levels in Female Pikeperch Sander lucioperca Treated with Poly(lactic-co-glycolic acid) Microparticles

Popis výsledku anglicky

Simple Summary Pikeperch Sander lucioperca is a promising candidate for intensive aquaculture. However, controlled reproduction has become the major bottleneck in pikeperch production. To improve and optimize its artificial reproduction, an effective method for hormone treatment is needed. The use of a poly(lactic-co-glycolic acid) microparticle-sustained-release system to administer gonadotropin-releasing hormone agonist to pikeperch resulted in acceptable reproductive output. Our results establish the potential of poly(lactic-co-glycolic acid) microparticle as a novel tool for hormone treatment in fish. Pikeperch Sander lucioperca is a piscivorous species considered a promising candidate for the diversification of intensive aquaculture. This study aimed to determine the effect of a sustained-release delivery system incorporating mammalian gonadotropin-releasing hormone agonist (mGnRHa) into poly(lactic-co-glycolic acid) (PLGA) microparticles on the sex steroid levels and aspects of artificial reproduction of pikeperch. Fish were divided into four groups and injected with 20 mu g mGnRHa/kg, 5-day release microparticles encapsulated with 5 mu g GnRHa/kg BW (PLGA 5), 20 mu g GnRHa/kg (PLGA 20), or 1 mL/kg 0.9% NaCl (control). Cumulative percentage ovulation was 100% in the PLGA 5 group, significantly higher than in other tested groups. No differences among groups were observed in latency or fecundity. The level of 11-ketotestosterone (11-KT) peaked at 40 h post-injection, and was sustained during ovulation, in all treated groups. The 17 beta-estradiol (E2) concentration increased in the mGnRHa-only group immediately after hormone injection, while both PLGA groups showed a reduction in E2 after injection, continuing to decrease until ovulation. A low dose of mGnRHa in PLGA microparticles significantly improves induction of ovulation and results in acceptable reproductive performance, which may positively affect pikeperch production under controlled conditions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/QK1810221" target="_blank" >QK1810221: Využití mikročástic jako nosičů hormonálně aktivních látek v řízené reprodukci ryb.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ANIMALS

ISSN

2076-2615

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

1-11

Kód UT WoS článku

000749805300001

EID výsledku v databázi Scopus

2-s2.0-85122875266