Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14160%2F23%3A00134605" target="_blank" >RIV/00216224:14160/23:00134605 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water

Popis výsledku v původním jazyce

Nerve agents (soman, sarin, tabun, VX), organophosphate (parathion, malathion) and carbamate (aldicarb) pesticides are commonly referred to as cholinesterase inhibitors (ChI), which are substances with very high toxicity. A fast, cheap, and sensitive sensor is needed to detect ChI as quickly as possible in order to carry out the appropriate treatment for those affected. Detection tubes (DTs) that use pellets with immobilized cholinesterase for colorimetric detection of the ChI presence, are just the type of detector that meets the above-mentioned requirements. Moreover, enzyme-based biosensors such as DTs have a detection limit between 0.005–0.01 mg∙m−3, which is the lowest in comparison with more expensive and unportable detectors using GC-MS, FTIR or IMS. DTs are based on fast enzymatic Ellman reaction. However, it suffers from an indistinct color transition from white to yellow in the absence of ChI. In this study, an innovative carrier in the form of coated matrix pellets was prepared utilizing the advantages of the previous experiment. The pellet cores contained the unique sorbent – Neusilin® US2 and the semipermeable coating consisted mainly of Eudragit® RL. The main aim of the experiment was to evaluate reaction rate, sensitivity, and color transition intensity of prepared samples in comparison with previous types of carriers.

Název v anglickém jazyce

Eudragit® RL coated pellets with immobilized butyrylcholinesterase for rapid detection of cholinesterase inhibitors in water

Popis výsledku anglicky

Nerve agents (soman, sarin, tabun, VX), organophosphate (parathion, malathion) and carbamate (aldicarb) pesticides are commonly referred to as cholinesterase inhibitors (ChI), which are substances with very high toxicity. A fast, cheap, and sensitive sensor is needed to detect ChI as quickly as possible in order to carry out the appropriate treatment for those affected. Detection tubes (DTs) that use pellets with immobilized cholinesterase for colorimetric detection of the ChI presence, are just the type of detector that meets the above-mentioned requirements. Moreover, enzyme-based biosensors such as DTs have a detection limit between 0.005–0.01 mg∙m−3, which is the lowest in comparison with more expensive and unportable detectors using GC-MS, FTIR or IMS. DTs are based on fast enzymatic Ellman reaction. However, it suffers from an indistinct color transition from white to yellow in the absence of ChI. In this study, an innovative carrier in the form of coated matrix pellets was prepared utilizing the advantages of the previous experiment. The pellet cores contained the unique sorbent – Neusilin® US2 and the semipermeable coating consisted mainly of Eudragit® RL. The main aim of the experiment was to evaluate reaction rate, sensitivity, and color transition intensity of prepared samples in comparison with previous types of carriers.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/VI20192022172" target="_blank" >VI20192022172: Detekční trubičky druhé generace pro kontinuální a opakované monitorování nervově paralytických a jiných toxických látek</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů