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Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors Dvojitĕ obalené pelety se semipermeabilním obalem z ethylcelulosy určené k detekci inhibitorů cholinesteras

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F20%3A00365176" target="_blank" >RIV/68407700:21460/20:00365176 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://www.prolekare.cz/casopisy/ceska-slovenska-farmacie/2020-1-24/double-coated-pellets-with-semipermeable-ethylcellulose-coating-for-detection-of-cholinesterase-inhibitors-122472" target="_blank" >https://www.prolekare.cz/casopisy/ceska-slovenska-farmacie/2020-1-24/double-coated-pellets-with-semipermeable-ethylcellulose-coating-for-detection-of-cholinesterase-inhibitors-122472</a>

  • DOI - Digital Object Identifier

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors Dvojitĕ obalené pelety se semipermeabilním obalem z ethylcelulosy určené k detekci inhibitorů cholinesteras

  • Popis výsledku v původním jazyce

    Currently, nerve agents are often used in terrorist attacks or assassinations. In such cases, it is necessary to detect them quickly, accurately and easily right in the field. Detection tubes, which are small devices containing pellets with immobilized cholinesterase and detection reagents, meet these conditions. Their detection mechanism is based on a highly sensitive enzymatic Ellman reaction, when in the absence of cholinesterase inhibitors the pellets develop a visible yellow color, whereas in their presence the carriers retain the original color. The rate of reaction, its sensitivity and the distinct color transition are the key points of the research. In this experiment, double-coated pellets were prepared. The first coating contained the butyrylcholinesterase immobilized in hypromellose, while the second coating consisted of ethylcellulose and triethyl citrate. Based on the properties of such carriers, samples containing lactose dispersed in the ethylcellulose coating were also prepared, which was expected to have an effect on increasing the permeability of the coating and hence the detection rate and color intensity. In addition to selected physicochemical properties, carriers were evaluated for enzyme activity, sensitivity and color transition intensity. Samples showing the best properties were subjected to a 24-months stability test at three different temperatures and humidity.

  • Název v anglickém jazyce

    Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors Dvojitĕ obalené pelety se semipermeabilním obalem z ethylcelulosy určené k detekci inhibitorů cholinesteras

  • Popis výsledku anglicky

    Currently, nerve agents are often used in terrorist attacks or assassinations. In such cases, it is necessary to detect them quickly, accurately and easily right in the field. Detection tubes, which are small devices containing pellets with immobilized cholinesterase and detection reagents, meet these conditions. Their detection mechanism is based on a highly sensitive enzymatic Ellman reaction, when in the absence of cholinesterase inhibitors the pellets develop a visible yellow color, whereas in their presence the carriers retain the original color. The rate of reaction, its sensitivity and the distinct color transition are the key points of the research. In this experiment, double-coated pellets were prepared. The first coating contained the butyrylcholinesterase immobilized in hypromellose, while the second coating consisted of ethylcellulose and triethyl citrate. Based on the properties of such carriers, samples containing lactose dispersed in the ethylcellulose coating were also prepared, which was expected to have an effect on increasing the permeability of the coating and hence the detection rate and color intensity. In addition to selected physicochemical properties, carriers were evaluated for enzyme activity, sensitivity and color transition intensity. Samples showing the best properties were subjected to a 24-months stability test at three different temperatures and humidity.

Klasifikace

  • Druh

    J<sub>SC</sub> - Článek v periodiku v databázi SCOPUS

  • CEP obor

  • OECD FORD obor

    30108 - Toxicology

Návaznosti výsledku

  • Projekt

  • Návaznosti

    N - Vyzkumna aktivita podporovana z neverejnych zdroju

Ostatní

  • Rok uplatnění

    2020

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Česká a slovenská farmacie

  • ISSN

    1210-7816

  • e-ISSN

  • Svazek periodika

    69

  • Číslo periodika v rámci svazku

    1

  • Stát vydavatele periodika

    CZ - Česká republika

  • Počet stran výsledku

    9

  • Strana od-do

    24-32

  • Kód UT WoS článku

  • EID výsledku v databázi Scopus

    2-s2.0-85085539451