Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00002296" target="_blank" >RIV/00216224:14310/00:00002296 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic
Popis výsledku v původním jazyce
The aim of this study was to estimate the genetic variability of 21 Staphylococcus epidermidis, 20 Staphylococcus haemolyticus and 30 Staphylococcus hominis strains resistant to oxacillin and multiresistant to antibiotics isolated from various clinical materials in Czech Republic during 1996-1999. Identification of these strains was carried out by estimating their biochemical features by use of STAPHYTest 16 and the ID32 Staph systems. In all the strains the resistance to 15 antibiotics was tested. Thephage-typing was performed in using the Lyon and London phage sets at 100xRTD as well as the polyvalent phages and their host-range mutants. Oxacillin resistance was confirmed by the gene mecA PCR-amplification. For purpose of estimating genetic relatio nships in all the strains under study the SmaI-macrorestriction analysis, ribotyping, plasmid content and PCR-amplification of spacer region in 16S-23S rDNA were carried out. The results of SmaI-macrorestriction analysis in S. epidermidis
Název v anglickém jazyce
Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic
Popis výsledku anglicky
The aim of this study was to estimate the genetic variability of 21 Staphylococcus epidermidis, 20 Staphylococcus haemolyticus and 30 Staphylococcus hominis strains resistant to oxacillin and multiresistant to antibiotics isolated from various clinical materials in Czech Republic during 1996-1999. Identification of these strains was carried out by estimating their biochemical features by use of STAPHYTest 16 and the ID32 Staph systems. In all the strains the resistance to 15 antibiotics was tested. Thephage-typing was performed in using the Lyon and London phage sets at 100xRTD as well as the polyvalent phages and their host-range mutants. Oxacillin resistance was confirmed by the gene mecA PCR-amplification. For purpose of estimating genetic relatio nships in all the strains under study the SmaI-macrorestriction analysis, ribotyping, plasmid content and PCR-amplification of spacer region in 16S-23S rDNA were carried out. The results of SmaI-macrorestriction analysis in S. epidermidis
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA301%2F99%2FD075" target="_blank" >GA301/99/D075: Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2000
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
9th International Symposium on Staphylococci and Staphylococcal Infections
ISBN
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ISSN
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e-ISSN
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Počet stran výsledku
1
Strana od-do
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Název nakladatele
JDS
Místo vydání
Kolding
Místo konání akce
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Datum konání akce
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Typ akce podle státní příslušnosti
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Kód UT WoS článku
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