Aktualizace cherakteristiky fenotypu a genotypu kmenů Staphylococcus hominis subsp. novobiosepticus v českých nemocnicích

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00011627" target="_blank" >RIV/00216224:14310/04:00011627 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Update on the genotypic and phenotypic characteristics of <I>Staphylococcus hominis</i> subsp. <I>novobiosepticus</i> strains from Czech hospitals

Popis výsledku v původním jazyce

A new subspecies, Staphylococcus hominis subsp. novobiosepticus (SHN), isolated from relevant human clinical material, was described in 1998, arousing little attention in the world. We present genetic and phenotypic characteristics of SHN strains from 25Czech cities. The aims of the present study were to determine phenotypic variability of SHN strains and to compare different molecular techniques for suitability for routine discrimination between the methicillin-resistant strains of S. hominis subsp. hominis and SHN. A total of 269 SHN strains collected in 30 Czech hospitals mainly from the blood (69%) were characterized phenotypically by both conventional tests and commercial kits and tested for resistance to 12 antibiotics. The following molecular typing methods were used to map characteristics of 90 selected SHN strains: SmaI macrorestriction analysis resolved by PFGE; amplification of rDNA intergenic spacers (ITS-PCR); AP-PCR; rep-PCR with primer ERIC2; and PCR-RFLP analysis of me

Název v anglickém jazyce

Update on the genotypic and phenotypic characteristics of <I>Staphylococcus hominis</i> subsp. <I>novobiosepticus</i> strains from Czech hospitals

Popis výsledku anglicky

A new subspecies, Staphylococcus hominis subsp. novobiosepticus (SHN), isolated from relevant human clinical material, was described in 1998, arousing little attention in the world. We present genetic and phenotypic characteristics of SHN strains from 25Czech cities. The aims of the present study were to determine phenotypic variability of SHN strains and to compare different molecular techniques for suitability for routine discrimination between the methicillin-resistant strains of S. hominis subsp. hominis and SHN. A total of 269 SHN strains collected in 30 Czech hospitals mainly from the blood (69%) were characterized phenotypically by both conventional tests and commercial kits and tested for resistance to 12 antibiotics. The following molecular typing methods were used to map characteristics of 90 selected SHN strains: SmaI macrorestriction analysis resolved by PFGE; amplification of rDNA intergenic spacers (ITS-PCR); AP-PCR; rep-PCR with primer ERIC2; and PCR-RFLP analysis of me

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F02%2F1505" target="_blank" >GA301/02/1505: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

11th International Symposium on Staphylococci & Staphylococcal Infections. Plenary Summaries & Poster Abstracts

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

149

Název nakladatele

Medical University of South Carolina

Místo vydání

Charleston, South Carolina, USA

Místo konání akce

Charleston, South Carolina, USA

Datum konání akce

24. 10. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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