Charakterizace kmenů <I>Staphylococcus equorum</I> a <I>Staphylococcus succinus</i> izolovaných z klinického materiálu humánního původu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00015634" target="_blank" >RIV/00216224:14310/06:00015634 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

<I>Staphylococcus equorum</I> and <I>Staphylococcus succinus</I> isolated from human clinical specimens

Popis výsledku v původním jazyce

A polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. Phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as Staphylococcus xylosus and three strains as ambiguous S. xylosus/ Staphylococcus equorum. In contrast to biotyping, ribotyping with EcoRI and HindIII and wholle-cell protein fingerprinting assigned six analysed strains as S. equorum, and five strains as Staphylococcus succinus. Confirmation of the identification was done by partial 16S rRNA gene sequencing and S. equorum isolates were verified by a previously described PCR assay targeting the sodA gene. From the data it has been implied that ribotyping and whole-cell protein analysis can be used to differentiate between the biochemically almost indistinguishable species S.xylosus, S. equorum or S. succinus. The present study confirms what is believed to be first occurrence of S. equorum strains

Název v anglickém jazyce

<I>Staphylococcus equorum</I> and <I>Staphylococcus succinus</I> isolated from human clinical specimens

Popis výsledku anglicky

A polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. Phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as Staphylococcus xylosus and three strains as ambiguous S. xylosus/ Staphylococcus equorum. In contrast to biotyping, ribotyping with EcoRI and HindIII and wholle-cell protein fingerprinting assigned six analysed strains as S. equorum, and five strains as Staphylococcus succinus. Confirmation of the identification was done by partial 16S rRNA gene sequencing and S. equorum isolates were verified by a previously described PCR assay targeting the sodA gene. From the data it has been implied that ribotyping and whole-cell protein analysis can be used to differentiate between the biochemically almost indistinguishable species S.xylosus, S. equorum or S. succinus. The present study confirms what is believed to be first occurrence of S. equorum strains

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Medical Microbiology

ISSN

0022-2615

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

523-528

Kód UT WoS článku

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EID výsledku v databázi Scopus

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