Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00012492" target="_blank" >RIV/00216224:14310/05:00012492 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles.

Popis výsledku v původním jazyce

Staphylococcus xylosus and Staphylococcus equorum are two biochemically and phylogenetically close species. Athough S.xylosus is usually only transient on human and is primarily acquired from domestic animal and their products, it has been isolated alsofrom human clinical specimens, mainly from urinary tract infection. Lack of the biochemical key tests makes differentiating of these species difficult. It is very important to find another reliable approach to distinguish S.xylosus and S.equorum species.A group of 18 presumptive S.xylosus strains from human clinical isolates were analysed by biochemical tests and ribotyping. SDS-PAGE protein analysis was performed to confirm the results of ribotyping. Biochemical properties were tested by API Staph andID 32 STAPH commercial kits as well as by conventional tests. Ribotyping was done with EcoRI and HindIII restriction enzymes and a probe complementary to 16S-23S rRNA.

Název v anglickém jazyce

Characterization of presumptive Staphylococcus xylosus strains by SDS-PAGE protein profiles.

Popis výsledku anglicky

Staphylococcus xylosus and Staphylococcus equorum are two biochemically and phylogenetically close species. Athough S.xylosus is usually only transient on human and is primarily acquired from domestic animal and their products, it has been isolated alsofrom human clinical specimens, mainly from urinary tract infection. Lack of the biochemical key tests makes differentiating of these species difficult. It is very important to find another reliable approach to distinguish S.xylosus and S.equorum species.A group of 18 presumptive S.xylosus strains from human clinical isolates were analysed by biochemical tests and ribotyping. SDS-PAGE protein analysis was performed to confirm the results of ribotyping. Biochemical properties were tested by API Staph andID 32 STAPH commercial kits as well as by conventional tests. Ribotyping was done with EcoRI and HindIII restriction enzymes and a probe complementary to 16S-23S rRNA.

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F02%2F1505" target="_blank" >GA301/02/1505: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

IX. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků.

ISBN

80-210-3635-4

ISSN

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e-ISSN

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Počet stran výsledku

27

Strana od-do

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Název nakladatele

Masarykova universita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

1. 1. 2005

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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