Ribotypizace a biotypizace kmenů druhové skupiny Staphylococcus sciuri humánního původu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00011628" target="_blank" >RIV/00216224:14310/04:00011628 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Ribotyping and biotyping of <I>Staphylococcus sciuri</I> species group from humans

Popis výsledku v původním jazyce

Members of the Staphylococcus sciuri species group are infrequently isolated from human clinical material. In our study a collection of 72 clinical isolates originating from Czech Republic and 6 reference type strains of S. sciuri complex were characterized by ribotyping and biotyping. All strains were ribotyped using EcoRI restriction enzyme and probe complementary to 16S and 23S rRNA. The relatedness of ribotypes was evaluated by using the Dice coefficient and clustered by UPGMA method. Biotyping wasperformed with the API Staph and ID 32 Staph kits supplemented by conventional tests key for this bacterial group and by whole-cell protein profiles analysis (1D SDS-PAGE). Biochemical identification of isolates from S. sciuri species group based on commercial kits was often uncertain due to variations observed in some tests and required additional testing. All isolates and type strains were clearly distinguished by ribotyping according to their specific ribotypes at the species or subsp

Název v anglickém jazyce

Ribotyping and biotyping of <I>Staphylococcus sciuri</I> species group from humans

Popis výsledku anglicky

Members of the Staphylococcus sciuri species group are infrequently isolated from human clinical material. In our study a collection of 72 clinical isolates originating from Czech Republic and 6 reference type strains of S. sciuri complex were characterized by ribotyping and biotyping. All strains were ribotyped using EcoRI restriction enzyme and probe complementary to 16S and 23S rRNA. The relatedness of ribotypes was evaluated by using the Dice coefficient and clustered by UPGMA method. Biotyping wasperformed with the API Staph and ID 32 Staph kits supplemented by conventional tests key for this bacterial group and by whole-cell protein profiles analysis (1D SDS-PAGE). Biochemical identification of isolates from S. sciuri species group based on commercial kits was often uncertain due to variations observed in some tests and required additional testing. All isolates and type strains were clearly distinguished by ribotyping according to their specific ribotypes at the species or subsp

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F02%2F1505" target="_blank" >GA301/02/1505: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

11th International Symposium on Staphylococci & Staphylococcal Infections. Plenary Summaries & Poster Abstracts

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

153

Název nakladatele

Medical University of South Carolina

Místo vydání

Charleston, South Carolina, USA

Místo konání akce

Charleston, South Carolina, USA

Datum konání akce

24. 10. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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