Genomic and phenotypic variability of multiresistant strains <I>Staphylococcus hominis</I> subsp. <I>novobiosepticus</I>

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00006632" target="_blank" >RIV/00216224:14310/02:00006632 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genomic and phenotypic variability of multiresistant strains <I>Staphylococcus hominis</I> subsp. <I>novobiosepticus</I>

Popis výsledku v původním jazyce

Background.In 1998,a new subspecies,Staphylococcus hominis subsp.novobiosepticus (SHN),isolated from human clinical material,was described by Kloos,et al.We present genetic and phenotypic characteristics of 192 SHN strains,isolated in 24 cities of the Czech Republic (CR). Methods.A total of 192 SHN strains were collected in 29 Czech hospitals mainly from the blood (72%).They were tested for resistance to 12 antibiotics by the disk-diffusion method.Molecular typing was carried out to map characteristicsof 70 selected SHN strains.To study possible genetic relatedness between strains,the Sma I- and Ksp I-macrorestriction analysis,plasmid content analysis and AP-PCR were used. Results.Multiresistant and virulent strains showed practically identical phenotypic characteristics as type strain CCM 4786 T .The most refined genotyping results were obtained by macrorestriction analysis,differentiating unambiguously between SHN and other S.hominis subspecies at a macrorestricton pattern similarit

Název v anglickém jazyce

Genomic and phenotypic variability of multiresistant strains <I>Staphylococcus hominis</I> subsp. <I>novobiosepticus</I>

Popis výsledku anglicky

Background.In 1998,a new subspecies,Staphylococcus hominis subsp.novobiosepticus (SHN),isolated from human clinical material,was described by Kloos,et al.We present genetic and phenotypic characteristics of 192 SHN strains,isolated in 24 cities of the Czech Republic (CR). Methods.A total of 192 SHN strains were collected in 29 Czech hospitals mainly from the blood (72%).They were tested for resistance to 12 antibiotics by the disk-diffusion method.Molecular typing was carried out to map characteristicsof 70 selected SHN strains.To study possible genetic relatedness between strains,the Sma I- and Ksp I-macrorestriction analysis,plasmid content analysis and AP-PCR were used. Results.Multiresistant and virulent strains showed practically identical phenotypic characteristics as type strain CCM 4786 T .The most refined genotyping results were obtained by macrorestriction analysis,differentiating unambiguously between SHN and other S.hominis subspecies at a macrorestricton pattern similarit

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F02%2F1505" target="_blank" >GA301/02/1505: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

10th International Symposium on Staphylococci and Staphylococcal Infections

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

65

Název nakladatele

Juntendo University

Místo vydání

Tsukuba, Japan

Místo konání akce

October 16-19, 2002

Datum konání akce

1. 1. 2002

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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