Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from 44 kb SmaI-restriction fragment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00002297" target="_blank" >RIV/00216224:14310/00:00002297 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from 44 kb SmaI-restriction fragment

Popis výsledku v původním jazyce

The primers were prepared for PCR-amplification of a part genomic sequence specific to Staphylococcus aureus strains. They were derived from the 44 kb SmaI-restriction fragment of S. aureus genomic DNA (SmaI fragment L on the restriction map of strain NCTC 8325) common to all strains of this species (Pantucek et al. 1996, Int. J. Syst. Bacteriol. 46, 216-222). By hybridization of the labelled 44 kb SmaI-fragment to EcoRI-restriction patterns of the genomic DNA of 13 strains representative of S. aureus subsp. aureus the 2052 bp EcoRI-subfragment was proved to be common to all the tested strains. This fragment was used as the hybridization probe the specificity of which for the species S. aureus was tested in 41 species and subspecies of the genus Staphylococcus as well as in 7 strains of related bacterial genera. The probe hybridized only to the DNA of S. aureus subsp. aureus strains and S. aureus subsp. anaerobius. No hybridization was observed to the DNA of other staphylococcal as wel

Název v anglickém jazyce

Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from 44 kb SmaI-restriction fragment

Popis výsledku anglicky

The primers were prepared for PCR-amplification of a part genomic sequence specific to Staphylococcus aureus strains. They were derived from the 44 kb SmaI-restriction fragment of S. aureus genomic DNA (SmaI fragment L on the restriction map of strain NCTC 8325) common to all strains of this species (Pantucek et al. 1996, Int. J. Syst. Bacteriol. 46, 216-222). By hybridization of the labelled 44 kb SmaI-fragment to EcoRI-restriction patterns of the genomic DNA of 13 strains representative of S. aureus subsp. aureus the 2052 bp EcoRI-subfragment was proved to be common to all the tested strains. This fragment was used as the hybridization probe the specificity of which for the species S. aureus was tested in 41 species and subspecies of the genus Staphylococcus as well as in 7 strains of related bacterial genera. The probe hybridized only to the DNA of S. aureus subsp. aureus strains and S. aureus subsp. anaerobius. No hybridization was observed to the DNA of other staphylococcal as wel

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F99%2FD075" target="_blank" >GA301/99/D075: Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

9th International Symposium on Staphylococci and Staphylococcal Infections

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

JDS

Místo vydání

Kolding

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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