Identification of <I>Staphylococcus aureus</I> based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb <I>Sma</I>I restriction fragment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00004693" target="_blank" >RIV/00216224:14310/01:00004693 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of <I>Staphylococcus aureus</I> based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb <I>Sma</I>I restriction fragment

Popis výsledku v původním jazyce

Primers were designed for polymerase chain reaction (PCR)-amplification of a genomic sequence specific to Staphylococcus aureus strains. The sequence corresponds to a part of the 44-kb Smal fragment (fragment L on the S. aureus NCTC 8325 restriction map)which was found to be common to strains of the S. aureus species (Pantucek et al., 1996, International journal of Systematic Bacteriology, 46: 216-222). The labelled 44-kb Smal restriction fragment derived from S. aureus NCTC 8325-4 was hybridized to the EcoRI restriction patterns of genomic DNA from 13 strains representing different macrorestriction types of S. aureus subsp. aureus. This made it possible to reveal the 2052 bp EcoRI restriction subfragment and to demonstrate its presence in all the tested strains. From the sequence of this subfragment, primers were designed by means of which the 826 bp amplicons were obtained in all 216 tested strains of S. aureus. No hybridization and PCR-products were observed in 40 collection strain

Název v anglickém jazyce

Identification of <I>Staphylococcus aureus</I> based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb <I>Sma</I>I restriction fragment

Popis výsledku anglicky

Primers were designed for polymerase chain reaction (PCR)-amplification of a genomic sequence specific to Staphylococcus aureus strains. The sequence corresponds to a part of the 44-kb Smal fragment (fragment L on the S. aureus NCTC 8325 restriction map)which was found to be common to strains of the S. aureus species (Pantucek et al., 1996, International journal of Systematic Bacteriology, 46: 216-222). The labelled 44-kb Smal restriction fragment derived from S. aureus NCTC 8325-4 was hybridized to the EcoRI restriction patterns of genomic DNA from 13 strains representing different macrorestriction types of S. aureus subsp. aureus. This made it possible to reveal the 2052 bp EcoRI restriction subfragment and to demonstrate its presence in all the tested strains. From the sequence of this subfragment, primers were designed by means of which the 826 bp amplicons were obtained in all 216 tested strains of S. aureus. No hybridization and PCR-products were observed in 40 collection strain

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F99%2FD075" target="_blank" >GA301/99/D075: Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Probes

ISSN

0890-8508

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

249

Kód UT WoS článku

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EID výsledku v databázi Scopus

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