Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00003324" target="_blank" >RIV/00216224:14310/00:00003324 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis

Popis výsledku v původním jazyce

Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative andhepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds present in the extract and to its caffeoylquinic derivatives. Micellar electrokinetic capillary chromatography (MECC) has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ion strength of the background electrolyte, SDS concentration and temperature. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best

Název v anglickém jazyce

Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis

Popis výsledku anglicky

Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative andhepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds present in the extract and to its caffeoylquinic derivatives. Micellar electrokinetic capillary chromatography (MECC) has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ion strength of the background electrolyte, SDS concentration and temperature. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA303%2F00%2FD062" target="_blank" >GA303/00/D062: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Slovenská chemická společnost

Místo vydání

Bratislava

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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