Determination of phenolic compounds in artichoke extract by means of MEKC

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00004276" target="_blank" >RIV/00216224:14310/01:00004276 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of phenolic compounds in artichoke extract by means of MEKC

Popis výsledku v původním jazyce

Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative andhepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds presented in the extract and to their caffeoylquinic derivatives. MEKC has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ionic strength of the background electrolyte, SDS concentration and temperature of capillary. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best separation of the caffeoylquinic

Název v anglickém jazyce

Determination of phenolic compounds in artichoke extract by means of MEKC

Popis výsledku anglicky

Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative andhepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds presented in the extract and to their caffeoylquinic derivatives. MEKC has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ionic strength of the background electrolyte, SDS concentration and temperature of capillary. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best separation of the caffeoylquinic

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA303%2F00%2FD062" target="_blank" >GA303/00/D062: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of abstract ICES 2001 An Electrophoretic Odessey

ISBN

—

ISSN

—

e-ISSN

—

Počet stran výsledku

1

Strana od-do

80

Název nakladatele

Emmezeta Congressi

Místo vydání

Verona

Místo konání akce

Verona

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—