Determination of the Kinetic Parameters of Rhodanese by Electrophoretically Mediated Microanalysis in a Partially Filled Capillary

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00004410" target="_blank" >RIV/00216224:14310/01:00004410 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of the Kinetic Parameters of Rhodanese by Electrophoretically Mediated Microanalysis in a Partially Filled Capillary

Popis výsledku v původním jazyce

Recently, a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed, electrophoretically mediated microanalysis (EMMA). In this technique, substrate(s) and enzyme are introduced in the capillary as distinct plugs, the first analyte injected being the one with the lower electrophoretic mobility. Upon the application of an electric field, the two zones interpenetrate due the differences in their electrophoretic mobilities. Enzymatic reaction takes place and theresultant reaction product(s) and the unreacted substrate(s) are electrophoretically transported towards detector, where they are individually detected. In this communication the EMMA technique in was applied to determine the kinetic parameters of rhodanese - temperature optimum, KM for thiosulfate and cyanide and type of kinetic mechanism of enzymatic reaction. Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was discovered in 1933 by Lang. It is responsible for the trans

Název v anglickém jazyce

Determination of the Kinetic Parameters of Rhodanese by Electrophoretically Mediated Microanalysis in a Partially Filled Capillary

Popis výsledku anglicky

Recently, a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed, electrophoretically mediated microanalysis (EMMA). In this technique, substrate(s) and enzyme are introduced in the capillary as distinct plugs, the first analyte injected being the one with the lower electrophoretic mobility. Upon the application of an electric field, the two zones interpenetrate due the differences in their electrophoretic mobilities. Enzymatic reaction takes place and theresultant reaction product(s) and the unreacted substrate(s) are electrophoretically transported towards detector, where they are individually detected. In this communication the EMMA technique in was applied to determine the kinetic parameters of rhodanese - temperature optimum, KM for thiosulfate and cyanide and type of kinetic mechanism of enzymatic reaction. Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was discovered in 1933 by Lang. It is responsible for the trans

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F00%2F0785" target="_blank" >GA525/00/0785: Acidifikační procesy v sulfidových odpadech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of abstract 2nd International symposium "Separations in BioSciences SBS 2001"

ISBN

80-7080-437-8

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

112

Název nakladatele

Czech Chemical Society

Místo vydání

Prague

Místo konání akce

Prague

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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