Electrophoretically mediated microanalysis of rhodanese

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00006537" target="_blank" >RIV/00216224:14310/02:00006537 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Electrophoretically mediated microanalysis of rhodanese

Popis výsledku v původním jazyce

Ten years ago a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed and developed, electrophoretically mediated microanalysis (EMMA). In this method, substrate(s) and enzyme are introduced into the capillary as distinct plugs, the first analyte injected being the one with the lower electrophoretic mobility. Upon the application of an electric field, the two zones interpenetrate due to the differences in their electrophoretic mobilities. Enzymatic reactiontakes place and the resultant reaction product(s) and the unreacted substrate(s) are electrophoretically transported towards detector, where they are individually detected. Since its discovery by Bao and Regnier the EMMA methodology has been utilized ina number of biochemical systems - for assays of enzyme activities, determinations of substrates, Michaelis constants, etc. In this work, the EMMA method was applied to determine the kinetic parameters of rhodanese. Rhodanese (thiosulfate:

Název v anglickém jazyce

Electrophoretically mediated microanalysis of rhodanese

Popis výsledku anglicky

Ten years ago a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed and developed, electrophoretically mediated microanalysis (EMMA). In this method, substrate(s) and enzyme are introduced into the capillary as distinct plugs, the first analyte injected being the one with the lower electrophoretic mobility. Upon the application of an electric field, the two zones interpenetrate due to the differences in their electrophoretic mobilities. Enzymatic reactiontakes place and the resultant reaction product(s) and the unreacted substrate(s) are electrophoretically transported towards detector, where they are individually detected. Since its discovery by Bao and Regnier the EMMA methodology has been utilized ina number of biochemical systems - for assays of enzyme activities, determinations of substrates, Michaelis constants, etc. In this work, the EMMA method was applied to determine the kinetic parameters of rhodanese. Rhodanese (thiosulfate:

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA525%2F00%2F0785" target="_blank" >GA525/00/0785: Acidifikační procesy v sulfidových odpadech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceeding of the 13th International Symposium on Capillary Electroseparation Techniques ITP 2002

ISBN

—

ISSN

—

e-ISSN

—

Počet stran výsledku

1

Strana od-do

75

Název nakladatele

University of Helsinky, Finland

Místo vydání

Helsinki

Místo konání akce

September 1-4, 2002 Helsinki, Finland

Datum konání akce

1. 1. 2002

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—