Partial characterisation of a colored metabolite of Acidithiobacillus ferrooxidans grown on elemental sulfur

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00004415" target="_blank" >RIV/00216224:14310/01:00004415 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Partial characterisation of a colored metabolite of Acidithiobacillus ferrooxidans grown on elemental sulfur

Popis výsledku v původním jazyce

The aim of the study was the physico-chemical characterisation of a violet-colored metabolite isolated from a culture of Acidithiobacillus ferrooxidans grown on elemental sulfur. The metabolite, which was bound to the surface of the bacterial cells, wasdetectable visually after prolonged culture incubation, corresponding to a pH change from about pH 4 to about pH 0.5. When extracted from the bacteria, the metabolite was found to be hydrophobic and insoluble in water. X-ray structure analysis, mass spectroscopy, and NMR were used to elucidate the chemical structure of this metabolite. The results of all these methods were consistent, and confirmed the existence of aromatic pyrene rings which were connected, probably via aliphatic chains, to cell exopolymers. The amount of the metabolite produced varied from culture to culture, though the conditions which promote its production are currently unknown. The proposed chemical structure of the colored metabolite indicated a possible role in

Název v anglickém jazyce

Partial characterisation of a colored metabolite of Acidithiobacillus ferrooxidans grown on elemental sulfur

Popis výsledku anglicky

The aim of the study was the physico-chemical characterisation of a violet-colored metabolite isolated from a culture of Acidithiobacillus ferrooxidans grown on elemental sulfur. The metabolite, which was bound to the surface of the bacterial cells, wasdetectable visually after prolonged culture incubation, corresponding to a pH change from about pH 4 to about pH 0.5. When extracted from the bacteria, the metabolite was found to be hydrophobic and insoluble in water. X-ray structure analysis, mass spectroscopy, and NMR were used to elucidate the chemical structure of this metabolite. The results of all these methods were consistent, and confirmed the existence of aromatic pyrene rings which were connected, probably via aliphatic chains, to cell exopolymers. The amount of the metabolite produced varied from culture to culture, though the conditions which promote its production are currently unknown. The proposed chemical structure of the colored metabolite indicated a possible role in

Druh

C - Kapitola v odborné knize

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F00%2F0785" target="_blank" >GA525/00/0785: Acidifikační procesy v sulfidových odpadech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Biohydrometallurgy: Fundamentals, Technology and Sustainable Development

ISBN

0 444 50623 3

Počet stran výsledku

5

Strana od-do

363

Počet stran knihy

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Název nakladatele

Elsevier

Místo vydání

Amsterdam

Kód UT WoS kapitoly

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