Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00005468" target="_blank" >RIV/00216224:14310/02:00005468 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography

Popis výsledku v původním jazyce

Micellar electrokinetic capillary chromatography (MEKC) has been developed as a promising method for the determination of lignans in plant samples. The separation conditions have been optimised with respect to the different parameters including SDS and acetonitrile concentration, pH of the background electrolyte, separation voltage and temperature of capillary. The background electrolyte consisting of 40 mM SDS and 35 % acetonitrile in 10 mM tetraborate buffer (pH 9.3) was found to be the most suitableelectrolyte for this analysis. The applied voltage of 28 kV (positive polarity) and the temperature of capillary 25 oC gave the best separation of lignans. The inter-day reproducibility of the peak areas and the migration times were below 2.0 %. The results of MEKC analyses were compared with those obtained by capillary electrochromatography (CEC) and reversed-phase high performance liquid chromatography (RP-HPLC). The possibilities of using this method for determination of lignans in dr

Název v anglickém jazyce

Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography

Popis výsledku anglicky

Micellar electrokinetic capillary chromatography (MEKC) has been developed as a promising method for the determination of lignans in plant samples. The separation conditions have been optimised with respect to the different parameters including SDS and acetonitrile concentration, pH of the background electrolyte, separation voltage and temperature of capillary. The background electrolyte consisting of 40 mM SDS and 35 % acetonitrile in 10 mM tetraborate buffer (pH 9.3) was found to be the most suitableelectrolyte for this analysis. The applied voltage of 28 kV (positive polarity) and the temperature of capillary 25 oC gave the best separation of lignans. The inter-day reproducibility of the peak areas and the migration times were below 2.0 %. The results of MEKC analyses were compared with those obtained by capillary electrochromatography (CEC) and reversed-phase high performance liquid chromatography (RP-HPLC). The possibilities of using this method for determination of lignans in dr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA303%2F00%2FD062" target="_blank" >GA303/00/D062: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

253

Kód UT WoS článku

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EID výsledku v databázi Scopus

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