Expression and purification of putative rhamnosyltransferase from Mycobacterium tuberculosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00007109" target="_blank" >RIV/00216224:14310/02:00007109 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression and purification of putative rhamnosyltransferase from Mycobacterium tuberculosis

Popis výsledku v původním jazyce

The resistance of Mycobacterium tuberculosis is related to an unusual and complex structure of mycobacterial cell wall envelope. The cell wall consists of a core composed of cross-linked peptidoglycan to which the heteropolysaccharide arabinogalactan isattached, the distal ends of which are almost esterified with mycolic acids (mAGP). The second of the major macromolecular compounds of mycobacterial cell wall is lipoarabinomannan (LAM) that has been considered as a key surface molecule in host-pathogeninteractions[1,2]. In contrary to the knowledge of the major cell wall constituents there is not much known about their biosynthesis pathways and glycosyltransferases (GT) involved. The global aim of the project is to understand the role of some key glycosyltransferases focusing on their structural analysis. Using various bioinformatics tools, some promising sequences in the M. tuberculosis genome were found. Presented work is focused on RfbE protein, a putative mycobacterial rhamnosylt

Název v anglickém jazyce

Expression and purification of putative rhamnosyltransferase from Mycobacterium tuberculosis

Popis výsledku anglicky

The resistance of Mycobacterium tuberculosis is related to an unusual and complex structure of mycobacterial cell wall envelope. The cell wall consists of a core composed of cross-linked peptidoglycan to which the heteropolysaccharide arabinogalactan isattached, the distal ends of which are almost esterified with mycolic acids (mAGP). The second of the major macromolecular compounds of mycobacterial cell wall is lipoarabinomannan (LAM) that has been considered as a key surface molecule in host-pathogeninteractions[1,2]. In contrary to the knowledge of the major cell wall constituents there is not much known about their biosynthesis pathways and glycosyltransferases (GT) involved. The global aim of the project is to understand the role of some key glycosyltransferases focusing on their structural analysis. Using various bioinformatics tools, some promising sequences in the M. tuberculosis genome were found. Presented work is focused on RfbE protein, a putative mycobacterial rhamnosylt

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00A016" target="_blank" >LN00A016: BIOMOLEKULÁRNÍ CENTRUM</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemické listy

ISSN

0009-2770

e-ISSN

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Svazek periodika

96

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

1

Strana od-do

433

Kód UT WoS článku

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EID výsledku v databázi Scopus

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