Glycosyltransferases from Mycobacterium tuberculosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00008576" target="_blank" >RIV/00216224:14310/02:00008576 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Glycosyltransferases from Mycobacterium tuberculosis

Popis výsledku v původním jazyce

A putative rhamnosyltransferase Rv3782 belongs to a group of glycosyltansferases (GTs) that are responsible for biosynthesis of cell wall envelope of Mycobacterium tuberculosis. Mycobacterial cell wall contains some unique sugar components that play a crucial role in host-pathogen interaction and is responsible for high pathogen resistance against common therapeutic agents. Thus these GTs are interesting as potential drug targets. In previous part of our research we selected some genes of putative GTs (using various bioinformatics tools applied to M. tuberculosis H37Rv genome sequence) that should be mostly of interest. At present study we have been focused on structural analysis of Rv3782. The main problem is to obtain a sufficient amount of soluble and pure protein for crystallization studies. Most of the expressed protein forms inclusion bodies, probably because of its toxicity for host cells, and there are additional losses caused by high GTs hydrofobicity that complicates its puri

Název v anglickém jazyce

Glycosyltransferases from Mycobacterium tuberculosis

Popis výsledku anglicky

A putative rhamnosyltransferase Rv3782 belongs to a group of glycosyltansferases (GTs) that are responsible for biosynthesis of cell wall envelope of Mycobacterium tuberculosis. Mycobacterial cell wall contains some unique sugar components that play a crucial role in host-pathogen interaction and is responsible for high pathogen resistance against common therapeutic agents. Thus these GTs are interesting as potential drug targets. In previous part of our research we selected some genes of putative GTs (using various bioinformatics tools applied to M. tuberculosis H37Rv genome sequence) that should be mostly of interest. At present study we have been focused on structural analysis of Rv3782. The main problem is to obtain a sufficient amount of soluble and pure protein for crystallization studies. Most of the expressed protein forms inclusion bodies, probably because of its toxicity for host cells, and there are additional losses caused by high GTs hydrofobicity that complicates its puri

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00A016" target="_blank" >LN00A016: BIOMOLEKULÁRNÍ CENTRUM</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

The 6th Workshop of Biochemists and Molecular Biologists

ISBN

80-210-3053-4

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

66

Název nakladatele

Masarykova univerzita

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

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Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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