Expression of putative mycobacterial glycosyltransferase Rv1526c

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00007111" target="_blank" >RIV/00216224:14310/02:00007111 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of putative mycobacterial glycosyltransferase Rv1526c

Popis výsledku v původním jazyce

At present, there can be seen a huge prevalence of tuberculosis all over the world. The most serious problem of its treatment arises specially from multidrugs resistant (MDR) forms of Mycobacterium tuberculosis patogen that are not possible to cure by conventional antimycobacterial therapies. Our research is focused on glycosyltransferases (GTs) from M. tuberculosis, enzymes that are responsible for biosynthesis of a cell wall envelope of this pathogen. They build some unique sugar components that playa crucial role in host-pathogen interactions. Using various bioinformatics tools applied to M. tuberculosis genome sequence, genes of some putative GTs were selected and cosmids carrying DNA of these GTs were obtained from Pasteur institute in Paris. Presented contribution is focused on putative rhamnosyltransferase Rv1526c. Generally, the most complicating point in preparation of GTs is low expression of these proteins in soluble form (proteins are toxic for expression host cells, proba

Název v anglickém jazyce

Expression of putative mycobacterial glycosyltransferase Rv1526c

Popis výsledku anglicky

At present, there can be seen a huge prevalence of tuberculosis all over the world. The most serious problem of its treatment arises specially from multidrugs resistant (MDR) forms of Mycobacterium tuberculosis patogen that are not possible to cure by conventional antimycobacterial therapies. Our research is focused on glycosyltransferases (GTs) from M. tuberculosis, enzymes that are responsible for biosynthesis of a cell wall envelope of this pathogen. They build some unique sugar components that playa crucial role in host-pathogen interactions. Using various bioinformatics tools applied to M. tuberculosis genome sequence, genes of some putative GTs were selected and cosmids carrying DNA of these GTs were obtained from Pasteur institute in Paris. Presented contribution is focused on putative rhamnosyltransferase Rv1526c. Generally, the most complicating point in preparation of GTs is low expression of these proteins in soluble form (proteins are toxic for expression host cells, proba

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00A016" target="_blank" >LN00A016: BIOMOLEKULÁRNÍ CENTRUM</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

The 18th Biochemical Congress

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

118

Název nakladatele

Slovenská spoločnosť pre biochémiu a molekulárnu biológiu pri SAV

Místo vydání

Slovensko

Místo konání akce

September 10 - 13, 2002, Stará Lesná, Slovakia

Datum konání akce

1. 1. 2002

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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