Electrochemical determination of glutathion and phytochelatins redox state

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical determination of glutathion and phytochelatins redox state

Popis výsledku v původním jazyce

Glutathione (L-g-glutamyl-L-cysteinyl-glycine, GSH) occurs in different species of animals, plants, and procaryotes. It is a starting material for the synthesis of some peptides and proteins such as phytochelatins (PCs). Glutathione and phytochelatins play a key role in the detoxication of heavy metals in plant cells. In addition, GSH is a powerful antioxidant. Electrochemical methods have been employed in analysis of GSH and PCs and also in the study of oxidation and reduction of the sulphur group in cystein residues (reduced -SH/oxidized -S-S-). We used voltammetric methods in both differential pulse and square wave modes to study GSH and PCs redox states on a hanging mercury drop electrode. If the oxidation of reduced forms of the peptides (GSH andPCs) was started at -0.8 V (vs. Ag/AgCl/3M KCl), two reversible anodic peaks at -0.45 and -0.30 V were obtained. The first peak corresponds to the oxidation of the sulfhydryl group and its height is proportional to the amount of oxidized

Název v anglickém jazyce

Electrochemical determination of glutathion and phytochelatins redox state

Popis výsledku anglicky

Glutathione (L-g-glutamyl-L-cysteinyl-glycine, GSH) occurs in different species of animals, plants, and procaryotes. It is a starting material for the synthesis of some peptides and proteins such as phytochelatins (PCs). Glutathione and phytochelatins play a key role in the detoxication of heavy metals in plant cells. In addition, GSH is a powerful antioxidant. Electrochemical methods have been employed in analysis of GSH and PCs and also in the study of oxidation and reduction of the sulphur group in cystein residues (reduced -SH/oxidized -S-S-). We used voltammetric methods in both differential pulse and square wave modes to study GSH and PCs redox states on a hanging mercury drop electrode. If the oxidation of reduced forms of the peptides (GSH andPCs) was started at -0.8 V (vs. Ag/AgCl/3M KCl), two reversible anodic peaks at -0.45 and -0.30 V were obtained. The first peak corresponds to the oxidation of the sulfhydryl group and its height is proportional to the amount of oxidized

Druh

D - Stať ve sborníku

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XVII th International Symposium on Bioelectrochemistry and Bioenergetics

ISBN

nebylo uděleno

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

251

Název nakladatele

The Bioelectrochemical Society

Místo vydání

Florence, Italy

Místo konání akce

Florence, Italy

Datum konání akce

19. 6. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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