Electrochemical determination of glutathione, phytochelatin and phytochelatin synthase activity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F19%3A43920907" target="_blank" >RIV/62156489:43510/19:43920907 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.biocap.ma/wp-content/uploads/2019/10/Book-of-abstract.pdf" target="_blank" >http://www.biocap.ma/wp-content/uploads/2019/10/Book-of-abstract.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical determination of glutathione, phytochelatin and phytochelatin synthase activity

Popis výsledku v původním jazyce

The synthesis of nanoparticles, especially silver (AgNPs), for various biomedicinal applications is a topical issue1. Free SH groups provide reducing properties, allowing binding of toxic compounds2,3. Glutathione (GSH) and phytochelatins (PCs) occurs in different species of animals, plants, and bacteria4,5. The study was aimed at monitoring the change in the level of thiol compounds in plants exposed to AgNPs. Experimental part. The absorbance spectra were recorded by a UV-VIS spectrophotometer. Determination of thiols by DPV was performed at Autolab. The chromatographic system coupled on-line to the VIS detector was used. The PCs activity was determined by Nakazawa&apos;s study6. Results and discussion. In experimental work, we focused on green synthesis, which uses the reduction potential of plant extracts to form AgNPs. Compounds present in plant extracts catalyze the formation of nanoparticles and modify their surface. Electrochemical methods have been employed in the analysis of GSH and PCs and also in the study of oxidation and reduction of the sulphur group in cysteine residues (a reduced -SH and oxidized -S-S- forms). When the oxidation of reduced forms of the peptides (GSH and PCs) was started at -0.8 V, two reversible anodic peaks at -0.45 and -0.30 V were obtained. In addition, the levels of thiol compounds in plants exposed to AgNPs were studied. The enzyme PCs synthase is activated by the presence of metal ions. GSH, PCs and PCs synthase levels varied depending on the applied concentration and exposure time. Conclusion. The introduction of the method for evaluating phytochelatin formation and the phytochelatin synthase catalytic activity will bring new insights into the evaluation of the AgNPs toxicity to cell cultures7.

Název v anglickém jazyce

Electrochemical determination of glutathione, phytochelatin and phytochelatin synthase activity

Popis výsledku anglicky

The synthesis of nanoparticles, especially silver (AgNPs), for various biomedicinal applications is a topical issue1. Free SH groups provide reducing properties, allowing binding of toxic compounds2,3. Glutathione (GSH) and phytochelatins (PCs) occurs in different species of animals, plants, and bacteria4,5. The study was aimed at monitoring the change in the level of thiol compounds in plants exposed to AgNPs. Experimental part. The absorbance spectra were recorded by a UV-VIS spectrophotometer. Determination of thiols by DPV was performed at Autolab. The chromatographic system coupled on-line to the VIS detector was used. The PCs activity was determined by Nakazawa&apos;s study6. Results and discussion. In experimental work, we focused on green synthesis, which uses the reduction potential of plant extracts to form AgNPs. Compounds present in plant extracts catalyze the formation of nanoparticles and modify their surface. Electrochemical methods have been employed in the analysis of GSH and PCs and also in the study of oxidation and reduction of the sulphur group in cysteine residues (a reduced -SH and oxidized -S-S- forms). When the oxidation of reduced forms of the peptides (GSH and PCs) was started at -0.8 V, two reversible anodic peaks at -0.45 and -0.30 V were obtained. In addition, the levels of thiol compounds in plants exposed to AgNPs were studied. The enzyme PCs synthase is activated by the presence of metal ions. GSH, PCs and PCs synthase levels varied depending on the applied concentration and exposure time. Conclusion. The introduction of the method for evaluating phytochelatin formation and the phytochelatin synthase catalytic activity will bring new insights into the evaluation of the AgNPs toxicity to cell cultures7.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/LTC18002" target="_blank" >LTC18002: Vývoj nových materiálů vhodných pro 3D tisk s antimikrobiálními vlastnostmi (3D ANTIMICROB)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů