HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00184621" target="_blank" >RIV/62156489:43210/10:00184621 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax

Popis výsledku v původním jazyce

If there would be free heavy metals occurring in the organism without control it initiates creation of number of dangerous species (ROS). These species are disturbing of homeostasis of organism by subsequent oxidation of essential cellular structures asnucleic acids. Animal and plant organism has both one efficient defending system which includes synthesis of glutathione (GSH). But plants are limited in case of escape from metal contaminated area. Therefore plant cell has mechanism includes synthesis of phytochelatins (PC) from GSH by phytochelatin synthase. PC's are able to immobilize heavy metal ion via number of thiol groups. We treated the plants of flax (Linum usativum) by various concentration of Cd(II) ions (50, 250, and 500mM) in our study. Subsequently we used our developed method for determination of oxidation stress as GSH/GSSG ratio and levels of PC-2,3,4 and 5. We observed that most synthesised PC under the highest concentration of Cd(II) treatment was PC-2,4 and 5.

Název v anglickém jazyce

HPLC with electrochemical detection for studying of synthesis of phytochelatins in cadmium treated flax

Popis výsledku anglicky

If there would be free heavy metals occurring in the organism without control it initiates creation of number of dangerous species (ROS). These species are disturbing of homeostasis of organism by subsequent oxidation of essential cellular structures asnucleic acids. Animal and plant organism has both one efficient defending system which includes synthesis of glutathione (GSH). But plants are limited in case of escape from metal contaminated area. Therefore plant cell has mechanism includes synthesis of phytochelatins (PC) from GSH by phytochelatin synthase. PC's are able to immobilize heavy metal ion via number of thiol groups. We treated the plants of flax (Linum usativum) by various concentration of Cd(II) ions (50, 250, and 500mM) in our study. Subsequently we used our developed method for determination of oxidation stress as GSH/GSSG ratio and levels of PC-2,3,4 and 5. We observed that most synthesised PC under the highest concentration of Cd(II) treatment was PC-2,4 and 5.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biochemical Technology

ISSN

0974-2328

e-ISSN

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Svazek periodika

2

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

2

Strana od-do

11-12

Kód UT WoS článku

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EID výsledku v databázi Scopus

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