Monitoring of phytochelatin synthase activity in presence of cadmium(ii) ions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F11%3A00183669" target="_blank" >RIV/62156489:43210/11:00183669 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of phytochelatin synthase activity in presence of cadmium(ii) ions

Popis výsledku v původním jazyce

The oxidative stress and its influence on live organism have been studied for several decades. Both animals and plants has various mechanisms to minimize of influence of oxidative stress nevertheless the mechanisms belongs to plants are little more advanced especially if we considering heavy metal ion affects. Simply said plant has limited options in case of escape from polluted areas in comprise with animals. One of most developed mechanism which plants have developed to be resistant against heavy metal ion affecting is producing of peptides called phytochelatines. The synthesis of phytochelatins is catalyzed by g-Glu-Cys dipeptidyl transpeptidase phytochelatin synthase (PCS). Aim of this work was to suggest new tool (high performance liquid chromatography coupled with electrochemical detector-HPLC-ED) for determination of the phytochelatin synthase activity. The optimized tool was subsequently used for study PCS activity in the tobacco BY-2 cells treated with different concentrations

Název v anglickém jazyce

Monitoring of phytochelatin synthase activity in presence of cadmium(ii) ions

Popis výsledku anglicky

The oxidative stress and its influence on live organism have been studied for several decades. Both animals and plants has various mechanisms to minimize of influence of oxidative stress nevertheless the mechanisms belongs to plants are little more advanced especially if we considering heavy metal ion affects. Simply said plant has limited options in case of escape from polluted areas in comprise with animals. One of most developed mechanism which plants have developed to be resistant against heavy metal ion affecting is producing of peptides called phytochelatines. The synthesis of phytochelatins is catalyzed by g-Glu-Cys dipeptidyl transpeptidase phytochelatin synthase (PCS). Aim of this work was to suggest new tool (high performance liquid chromatography coupled with electrochemical detector-HPLC-ED) for determination of the phytochelatin synthase activity. The optimized tool was subsequently used for study PCS activity in the tobacco BY-2 cells treated with different concentrations

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/GA522%2F07%2F0692" target="_blank" >GA522/07/0692: Studium tvorby thiolových sloučenin u rostlin - uplatnění při remediačních technologiích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Interdisciplinary Toxicology

ISSN

1337-6853

e-ISSN

—

Svazek periodika

4

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

2

Strana od-do

68-69

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—