Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008148" target="_blank" >RIV/00216224:14310/03:00008148 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor

Popis výsledku v původním jazyce

The electrochemical immunosensor for the detection of the herbicide acetochlor was developed. Initially, the feasibility of the immunosensor was verified using the standard ELISA technique employing the competitive assay format with immobilized acetochlor. The assay principle of the disposable immunochemical biosensor was similar. The screen-printed electrode system served as the transducer, acetochlor was covalently immobilized on the surface of the gold working electrode activated with a self-assembled monolayer of cystamine. Acetochlor was linked to this layer through S-acetylmercaptosuccinic anhydride (AMSA). A limited amount of anti-acetochlor polyclonal rabbit antibody in solution competed with the analyzed acetochlor and the secondary goat anti-rabbit antibody labeled with peroxidase was chosen for detection. The amperometric measurement of peroxidase activity was carried out using 5?aminosalicylic acid (ASA) and hydrogen peroxide as substrates. Successful regeneration of the se

Název v anglickém jazyce

Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor

Popis výsledku anglicky

The electrochemical immunosensor for the detection of the herbicide acetochlor was developed. Initially, the feasibility of the immunosensor was verified using the standard ELISA technique employing the competitive assay format with immobilized acetochlor. The assay principle of the disposable immunochemical biosensor was similar. The screen-printed electrode system served as the transducer, acetochlor was covalently immobilized on the surface of the gold working electrode activated with a self-assembled monolayer of cystamine. Acetochlor was linked to this layer through S-acetylmercaptosuccinic anhydride (AMSA). A limited amount of anti-acetochlor polyclonal rabbit antibody in solution competed with the analyzed acetochlor and the secondary goat anti-rabbit antibody labeled with peroxidase was chosen for detection. The amperometric measurement of peroxidase activity was carried out using 5?aminosalicylic acid (ASA) and hydrogen peroxide as substrates. Successful regeneration of the se

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F97%2F0924" target="_blank" >GA525/97/0924: Stopová analýza toxických PCB a pesticidů s využitím detekčních a separačních metod založených na protilátkové specifitě</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Environmental and Analytical Chemistry

ISSN

0306-7319

e-ISSN

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Svazek periodika

83

Číslo periodika v rámci svazku

7-8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

609-620

Kód UT WoS článku

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EID výsledku v databázi Scopus

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