Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008229" target="_blank" >RIV/00216224:14310/03:00008229 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/03:17033006

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica

Popis výsledku v původním jazyce

The bioaccumulation of heavy metals (cadmium, nickel, cobalt and zinc) and the effect of these metals on the production of metallothionein and metallothionein-like proteins (MT) in Yarrowia lipolytica was studied by electrochemical methods. The concentrations of heavy metals were determined by the differential pulse voltammetry (DPV). A combination of the constant current chronopotentiometric stripping analysis (CPSA) and adsorptive transfer stripping technique (AdTS) was used to determine the content of MT in cells. Both the bioaccumulation of heavy metals and the production of MT in different cell compartments of Yarrowia lipolytica exposed to heavy metals were monitored. The LD50 of each metal was determined from the number of viable cells in yeastcultures. The highest concentrations of heavy metals were found in the cell wall and membrane debris while the lowest concentrations were detected in cytoplasm. Cadmium and nickel showed the most significant effect on the production of MT

Název v anglickém jazyce

Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica

Popis výsledku anglicky

The bioaccumulation of heavy metals (cadmium, nickel, cobalt and zinc) and the effect of these metals on the production of metallothionein and metallothionein-like proteins (MT) in Yarrowia lipolytica was studied by electrochemical methods. The concentrations of heavy metals were determined by the differential pulse voltammetry (DPV). A combination of the constant current chronopotentiometric stripping analysis (CPSA) and adsorptive transfer stripping technique (AdTS) was used to determine the content of MT in cells. Both the bioaccumulation of heavy metals and the production of MT in different cell compartments of Yarrowia lipolytica exposed to heavy metals were monitored. The LD50 of each metal was determined from the number of viable cells in yeastcultures. The highest concentrations of heavy metals were found in the cell wall and membrane debris while the lowest concentrations were detected in cytoplasm. Cadmium and nickel showed the most significant effect on the production of MT

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

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Svazek periodika

neuveden

Číslo periodika v rámci svazku

60

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

29-36

Kód UT WoS článku

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EID výsledku v databázi Scopus

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