Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008231" target="_blank" >RIV/00216224:14310/03:00008231 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/03:17033157

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9

Popis výsledku v původním jazyce

Elimination voltammetry with linear scan (EVLS) has been applied to the resolution of reduction signals of adenine and cytosine in short synthetic homo-oligodeoxynucleotides (dA9 and dC9). In comparison with the common electrochemical methods (linear sweep, differential pulse, and square wave voltammetry) EVLS enables to resolve the overlapped signals by using the function which eliminates the charging and kinetic currents ( , ) and conserves the diffusion current ( ). For the adsorbed electroactive substance, this elimination function gives a well readable peak-counterpeak which has successfully been utilized to the analysis of overlapped reduction signals of adenine and cytosine on hanging mercury drop electrode (HMDE). The height and potential of signals studied were affected by the dC9/dA9 ratio, the time of accumulation, the stirring speed during the adsorption, and pH. Our results showed that EVLS in connection with the adsorption procedure is a useful tool for qualitative and qu

Název v anglickém jazyce

Application of Elimination Voltammetry to the Resolution of Adenine and Cytosine Signals in Oligonucleotides. I. Homo-oligodeoxynucleotides dA9 and dC9

Popis výsledku anglicky

Elimination voltammetry with linear scan (EVLS) has been applied to the resolution of reduction signals of adenine and cytosine in short synthetic homo-oligodeoxynucleotides (dA9 and dC9). In comparison with the common electrochemical methods (linear sweep, differential pulse, and square wave voltammetry) EVLS enables to resolve the overlapped signals by using the function which eliminates the charging and kinetic currents ( , ) and conserves the diffusion current ( ). For the adsorbed electroactive substance, this elimination function gives a well readable peak-counterpeak which has successfully been utilized to the analysis of overlapped reduction signals of adenine and cytosine on hanging mercury drop electrode (HMDE). The height and potential of signals studied were affected by the dC9/dA9 ratio, the time of accumulation, the stirring speed during the adsorption, and pH. Our results showed that EVLS in connection with the adsorption procedure is a useful tool for qualitative and qu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

1529-1535

Kód UT WoS článku

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EID výsledku v databázi Scopus

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