Aplikace eliminační voltametrie pro rozlišení signálů adeninu a cytosinu v oligonukleotidech. II. Hetero-oligodeoxynukleotidy s různou sekvencí adeninových a cytosinových nukleotidů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00016803" target="_blank" >RIV/00216224:14310/06:00016803 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/06:00047932

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides

Popis výsledku v původním jazyce

Elimination voltammetry with linear scan (EVLS) was applied to the resolution of reduction signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C. The EVLS evaluation required linear sweep voltammograms measured on a hanging mercury drop electrode (HMDE) at different scan rates. Compared to linear sweep voltammetry (LSV) and usual voltammetric methods the EVLS is capable of resolving the overlapped A and C signals, specifically by using the elimination function which eliminates the charging and kinetic currents and conserves the diffusion current. Since for an adsorbed electroactive substance this elimination function yields a well readable peak-counterpeak signal, theadsorptive stripping (AdS) procedure was favorably used. While on LSV curves the only one reduction peak of A and C residues was observed in all ODNs, the EVLS yielded two separated peaks in dependence on A, C sequences and pH. Our resul

Název v anglickém jazyce

Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides

Popis výsledku anglicky

Elimination voltammetry with linear scan (EVLS) was applied to the resolution of reduction signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C. The EVLS evaluation required linear sweep voltammograms measured on a hanging mercury drop electrode (HMDE) at different scan rates. Compared to linear sweep voltammetry (LSV) and usual voltammetric methods the EVLS is capable of resolving the overlapped A and C signals, specifically by using the elimination function which eliminates the charging and kinetic currents and conserves the diffusion current. Since for an adsorbed electroactive substance this elimination function yields a well readable peak-counterpeak signal, theadsorptive stripping (AdS) procedure was favorably used. While on LSV curves the only one reduction peak of A and C residues was observed in all ODNs, the EVLS yielded two separated peaks in dependence on A, C sequences and pH. Our resul

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

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Svazek periodika

18

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

662-669

Kód UT WoS článku

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EID výsledku v databázi Scopus

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