Development of piezoelectric immunosensors for competitive and direct determination of atrazine
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008696" target="_blank" >RIV/00216224:14310/03:00008696 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00000493:G41__/03:25150
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Development of piezoelectric immunosensors for competitive and direct determination of atrazine
Popis výsledku v původním jazyce
The improved highly sensitive piezoelectric immunosensor has been developed. The gold electrodes of the piezoelectric quartz crystals were modified with self-assembled layer of cysteamine and carboxyderivative of atrazine was covalently attached after activation with O-(N-succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate (TSTU). The competitive immunoassay for atrazine was developed using anti-atrazine monoclonal antibody (MAb) D6F3. The limit of detection for atrazine was 0.025 ng/ml, the total time of analysis being 25 min. The repeated use of the immunosensors was possible after regeneration using pepsin at pH 2.0. The direct immunosensor for atrazine was constructed by oriented immobilization of anti-atrazine MAb to Protein A covalentlyattached to the gold surface activated with 3,3'-dithio-bis(propionic acid N-hydroxysuccinimide ester) (DTSP). The MAb-Protein A complex was stabilized by cross-linking with dimethyl pimelimidate. Thus developed immunosensor was able to s
Název v anglickém jazyce
Development of piezoelectric immunosensors for competitive and direct determination of atrazine
Popis výsledku anglicky
The improved highly sensitive piezoelectric immunosensor has been developed. The gold electrodes of the piezoelectric quartz crystals were modified with self-assembled layer of cysteamine and carboxyderivative of atrazine was covalently attached after activation with O-(N-succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate (TSTU). The competitive immunoassay for atrazine was developed using anti-atrazine monoclonal antibody (MAb) D6F3. The limit of detection for atrazine was 0.025 ng/ml, the total time of analysis being 25 min. The repeated use of the immunosensors was possible after regeneration using pepsin at pH 2.0. The direct immunosensor for atrazine was constructed by oriented immobilization of anti-atrazine MAb to Protein A covalentlyattached to the gold surface activated with 3,3'-dithio-bis(propionic acid N-hydroxysuccinimide ester) (DTSP). The MAb-Protein A complex was stabilized by cross-linking with dimethyl pimelimidate. Thus developed immunosensor was able to s
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2003
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Sensors and Actuators B Chemical
ISSN
0925-4005
e-ISSN
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Svazek periodika
91
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
9
Strana od-do
333-341
Kód UT WoS článku
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EID výsledku v databázi Scopus
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