Differential effects of transcription coactivators CBP and p300 on v-myb-transfromed monoblasts

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Differential effects of transcription coactivators CBP and p300 on v-myb-transfromed monoblasts

Popis výsledku v původním jazyce

The v-myb oncogene is efficiently expressed in BM2 monoblasts thus blocking their terminal differentiation steps. Histone acetylation plays an important role in regulation of transcription and is particularly relevant to regulation and pathology of hematopoiesis. Recently, we have shown that inhibitor of histone deacetylases trichostatin A (TSA) causes histone hyperacetylation and differentiation of BM2 cells into macrophage polycaryons. In this work we used another approach to stimulate histone acetylation in BM2 cells. We prepared two variants of BM2 cells ectopically expressing two histone acetyl transferases CBP and p300. The expression of CBP as well as p300 itself did not affect the phenotype of BM2 cells, but it enhanced their response to the differentiation inducers TPA and TSA. Although CBP and p300 are closely related proteins, many differences in their function have been described. We noted differences in kinetics of differentiation induced by CBP and p300 in BM2 cells.

Název v anglickém jazyce

Differential effects of transcription coactivators CBP and p300 on v-myb-transfromed monoblasts

Popis výsledku anglicky

The v-myb oncogene is efficiently expressed in BM2 monoblasts thus blocking their terminal differentiation steps. Histone acetylation plays an important role in regulation of transcription and is particularly relevant to regulation and pathology of hematopoiesis. Recently, we have shown that inhibitor of histone deacetylases trichostatin A (TSA) causes histone hyperacetylation and differentiation of BM2 cells into macrophage polycaryons. In this work we used another approach to stimulate histone acetylation in BM2 cells. We prepared two variants of BM2 cells ectopically expressing two histone acetyl transferases CBP and p300. The expression of CBP as well as p300 itself did not affect the phenotype of BM2 cells, but it enhanced their response to the differentiation inducers TPA and TSA. Although CBP and p300 are closely related proteins, many differences in their function have been described. We noted differences in kinetics of differentiation induced by CBP and p300 in BM2 cells.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Memory in Living Systems

ISBN

80-210-3264-2

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

66

Název nakladatele

Czechoslovak Biological Society

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

19. 11. 2003

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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