Druhova identifikace evropskych diplozoonu (Diplozoidae, Monogenea) s pouzitim RFLP ITS2 rDNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00009908" target="_blank" >RIV/00216224:14310/04:00009908 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2)

Popis výsledku v původním jazyce

The second internal transcribed spacer (ITS2) of the ribosomal RNA genes of Diplozoon paradoxum and Paradiplozoon nagibinae were amplified and sequenced. The PCR product of D. paradoxum was bigger (840bp) than that of P. nagibinae (820bp). There was no intraspecific variability recorded in sequences from either species. Sequence comparisons and ITS2 RFLP pattern of eight European diplozoid species aimed to resolve their identification and amends the previous studies. Restriction fragment length polymorphism (RFLP) was used to distinguish both species from each other and from P. bliccae, P. homoion, P. megan, P. pavlovskii, P. sapae and Eudiplozoon nipponicum, using restriction enzymes AluI, HaeIII, HinfI, RsaI and SphI. The criteria for morphological identification of eight European diplozoids are also included with the main morphological characters of clamps, trapeze spur and anterior joining sclerites of eight diplozoid species being illustrated. Combination of the shape and comparis

Název v anglickém jazyce

Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2)

Popis výsledku anglicky

The second internal transcribed spacer (ITS2) of the ribosomal RNA genes of Diplozoon paradoxum and Paradiplozoon nagibinae were amplified and sequenced. The PCR product of D. paradoxum was bigger (840bp) than that of P. nagibinae (820bp). There was no intraspecific variability recorded in sequences from either species. Sequence comparisons and ITS2 RFLP pattern of eight European diplozoid species aimed to resolve their identification and amends the previous studies. Restriction fragment length polymorphism (RFLP) was used to distinguish both species from each other and from P. bliccae, P. homoion, P. megan, P. pavlovskii, P. sapae and Eudiplozoon nipponicum, using restriction enzymes AluI, HaeIII, HinfI, RsaI and SphI. The criteria for morphological identification of eight European diplozoids are also included with the main morphological characters of clamps, trapeze spur and anterior joining sclerites of eight diplozoid species being illustrated. Combination of the shape and comparis

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Parasitology

ISSN

0022-3395

e-ISSN

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Svazek periodika

90

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

817-822

Kód UT WoS článku

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EID výsledku v databázi Scopus

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