Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008781" target="_blank" >RIV/00216224:14310/03:00008781 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)

Popis výsledku v původním jazyce

The second internal transcribed spacer (ITS2) of ribosomal DNA of diplozoid parasites was amplified, sequenced and restriction enzyme digestion was performed to reveal restriction fragment length polymorphism (RFLP). Genetic markers for 8 species of European diplozoids were developed. As those diplozoids are very closely related, it was very difficult to find suitable enzymes to reveal different patterns for each species. Only a combination of digestion with AluI, HaeIII, HinfI, RsaI and SphI proved convenient to discriminate all species studied. Morphological analysis and measurements of the attachment apparatus of the studied species are also presented, together with comparison of the shape of trapeze spur and the anterior joining sclerites.

Název v anglickém jazyce

Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)

Popis výsledku anglicky

The second internal transcribed spacer (ITS2) of ribosomal DNA of diplozoid parasites was amplified, sequenced and restriction enzyme digestion was performed to reveal restriction fragment length polymorphism (RFLP). Genetic markers for 8 species of European diplozoids were developed. As those diplozoids are very closely related, it was very difficult to find suitable enzymes to reveal different patterns for each species. Only a combination of digestion with AluI, HaeIII, HinfI, RsaI and SphI proved convenient to discriminate all species studied. Morphological analysis and measurements of the attachment apparatus of the studied species are also presented, together with comparison of the shape of trapeze spur and the anterior joining sclerites.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Bulletin of the Scandinavian Society for Parasitology, Proceedings of the XXI. Symposium of the Scandinavian Socity for Parasitology, Bergen, Norway 12-15 June, 2003

ISBN

0803-4907

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

44-45

Název nakladatele

HS-Trykk A/S, Norway

Místo vydání

Bergen, Norway

Místo konání akce

Bergen, Norway

Datum konání akce

1. 1. 2003

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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