Vliv genů metylační dráhy na expresi genu FLC u Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00011539" target="_blank" >RIV/00216224:14310/04:00011539 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Influence of methylation pathway genes on FLC expression in Arabidopsis thaliana.

Popis výsledku v původním jazyce

Activity of FLC gene (Flowering Locus C) plays a central role in the control of flowering time in the autonomous and vernalization pathways. FLC activity is negatively regulated by other genes of both pathways. The main goal of this work was to assigne influence of the six late-flowering genes (dn, L4, L5, L6-1, L6 a Spi) of the new methylation pathway on the FLC expression. Also the effect of developmental stages, vernalization and 5-azacytidine treatment on the level of FLC mRNA in rosette leaves wasanalysed. Moreover the tissue distribution of FLC transcript was determined. For this purpose, reverse transcription and PCR amplification of FLC was used. All six late-flowering mutants of the methylation pathway increased level of FLC mRNA relative tocontrol lines S96 and Di-G. For analysis of the effect of developmental stages, vernalization and 5-azacytidine treatment the level of FLC mRNA was examined in rosette leaves, which were collected from plants (L4 and Spi) before and after

Název v anglickém jazyce

Influence of methylation pathway genes on FLC expression in Arabidopsis thaliana.

Popis výsledku anglicky

Activity of FLC gene (Flowering Locus C) plays a central role in the control of flowering time in the autonomous and vernalization pathways. FLC activity is negatively regulated by other genes of both pathways. The main goal of this work was to assigne influence of the six late-flowering genes (dn, L4, L5, L6-1, L6 a Spi) of the new methylation pathway on the FLC expression. Also the effect of developmental stages, vernalization and 5-azacytidine treatment on the level of FLC mRNA in rosette leaves wasanalysed. Moreover the tissue distribution of FLC transcript was determined. For this purpose, reverse transcription and PCR amplification of FLC was used. All six late-flowering mutants of the methylation pathway increased level of FLC mRNA relative tocontrol lines S96 and Di-G. For analysis of the effect of developmental stages, vernalization and 5-azacytidine treatment the level of FLC mRNA was examined in rosette leaves, which were collected from plants (L4 and Spi) before and after

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP521%2F02%2FP127" target="_blank" >GP521/02/P127: Interakce genů pro pozdní kvetení u Arabidopsis thaliana a jejich lokalizace na genetickou mapu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

15th International Conference on Arabidopsis Research

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

38-38

Název nakladatele

Satz Druck GmbH Teltow

Místo vydání

Potsdam

Místo konání akce

Berlin

Datum konání akce

11. 7. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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