Phytohormone profile and CiFL1 expression in young seedlings of Cichorium intybus L. var sativum exposed to high temperature in relation to vernalization and de-vernalization processes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00531697" target="_blank" >RIV/61389030:_____/20:00531697 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/20:73604702

Výsledek na webu

<a href="http://doi.org/10.1016/j.envexpbot.2020.104127" target="_blank" >http://doi.org/10.1016/j.envexpbot.2020.104127</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.envexpbot.2020.104127" target="_blank" >10.1016/j.envexpbot.2020.104127</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phytohormone profile and CiFL1 expression in young seedlings of Cichorium intybus L. var sativum exposed to high temperature in relation to vernalization and de-vernalization processes

Popis výsledku v původním jazyce

Cichorium intybus is a biennial plant species forming a taproot and a leaf rosette during the first year and which requires low temperature during vernalization to flower during the second year of its cycle. Heat stress, however, is known to induce premature flowering during the first year. The present work aims to compare the effect of heat treatment (38 °C during 3 days) and vernalization (4 °C during 6 weeks) on the phytohormonal profile and the expression of two chicory genes (CiFL1 and CiFT) homologous to the Arabidopsis flowering genes FLOWERING LOCUS C (FLC) and FLOWERING LOCUS T (FT). In Arabidopsis, FLC and FT code, respectively, for a repressor and an activator of flowering. Although CiFL1 was inhibited by vernalization, its expression was increased by heat treatment in both vernalized and non-vernalized seedlings while high temperature unexpectedly inhibited FT expression in vernalized seedlings. Vernalization induced a modification in the hormonal profile of C. intybus (cv. Melci) in a sense of a decrease in ethylene production, abscisic acid and total jasmonates content, while the level of salicylic and benzoic acids as well as polyamine spermidine increased. A limited effect on the gibberellins’ profile was observed. The subsequent impact of heat stress on phytohormone content was different in non-vernalized and vernalized seedlings, with a higher abscisic acid and jasmonates and a lower 1-aminocyclopropane-1-carboxylic acid concentration in the former than in the latter. These data are discussed in relation to the putative involvement of phytohormones in stress-induced flowering. It appears that heat stress implies different pathways than vernalization to hasten flowering process.

Název v anglickém jazyce

Phytohormone profile and CiFL1 expression in young seedlings of Cichorium intybus L. var sativum exposed to high temperature in relation to vernalization and de-vernalization processes

Popis výsledku anglicky

Cichorium intybus is a biennial plant species forming a taproot and a leaf rosette during the first year and which requires low temperature during vernalization to flower during the second year of its cycle. Heat stress, however, is known to induce premature flowering during the first year. The present work aims to compare the effect of heat treatment (38 °C during 3 days) and vernalization (4 °C during 6 weeks) on the phytohormonal profile and the expression of two chicory genes (CiFL1 and CiFT) homologous to the Arabidopsis flowering genes FLOWERING LOCUS C (FLC) and FLOWERING LOCUS T (FT). In Arabidopsis, FLC and FT code, respectively, for a repressor and an activator of flowering. Although CiFL1 was inhibited by vernalization, its expression was increased by heat treatment in both vernalized and non-vernalized seedlings while high temperature unexpectedly inhibited FT expression in vernalized seedlings. Vernalization induced a modification in the hormonal profile of C. intybus (cv. Melci) in a sense of a decrease in ethylene production, abscisic acid and total jasmonates content, while the level of salicylic and benzoic acids as well as polyamine spermidine increased. A limited effect on the gibberellins’ profile was observed. The subsequent impact of heat stress on phytohormone content was different in non-vernalized and vernalized seedlings, with a higher abscisic acid and jasmonates and a lower 1-aminocyclopropane-1-carboxylic acid concentration in the former than in the latter. These data are discussed in relation to the putative involvement of phytohormones in stress-induced flowering. It appears that heat stress implies different pathways than vernalization to hasten flowering process.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental and Experimental Botany

ISSN

0098-8472

e-ISSN

—

Svazek periodika

178

Číslo periodika v rámci svazku

Oct

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

104127

Kód UT WoS článku

000556869600003

EID výsledku v databázi Scopus

2-s2.0-85087979739